Molecular Devices FlexStation II, Operator'S Manual

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1.4 System Components
1.4.7 Consumables
The system consumables include:
•
Microplates
•
Pipette tips and racks
One rack of pipette tips is shipped with the system. For further information, see section
7.1, Parts and Accessories.
Caution: Do not use parts and accessories that are not authorized, specified by or
provided by Molecular Devices. Using unauthorized parts can damage the instrument.
Microplates
The instrument can accommodate standard 6, 12, 24, 48, 96 and 384-well microplates.
In Flex mode, however, you can only use 96- or 384-well formatted assay plates.
1
A
A1 A12
H1 H12
B
C
D
E
F
G
H
2 3 4 5 6 7 8 9 10 11 12
Top View of a 96-Well Microplate
For fluorescence, Molecular Devices generally recommends black-walled, clear-bottom
microplates for bottom reading, and all-black microplates for top reading, because they
have lower backgrounds than clear plates.
For luminescence, white microplates may optimize light collection.
Note: Not all microplates are made with the same materials. Some plastics, most notably
polystyrene, have significant native fluorescence and can cause moderate to sever
background fluorescence, especially in the UV range. If your fluorescence experiments
require
high sensitivity , it may be appropriate to use microplates designed and
designated by the manufacturer to reduce background fluorescence.
Pipette Tips and Racks
•
For 96-well assays, Molecular Devices specifies 200 µL Robbins brand, new-
generation Super Seal Autotips. For clear tips, use Molecular Devices PN 9000-0623.
•
For 384-well assays, Molecular Devices specifies 45 µL Lab Systems brand tips. For
clear tips, use Molecular Devices PN 9000-0512.
FlexStation II Operator’s Manual – Rev. D 17