3 Operating Procedures
4 Optional Excitation Scan with both Autofilters (Scan Figure 4)
In some Assays, a final excitation scan with the desired emission filter and the automatic
excitation filter will show additional background reduction. In the example (
Scan Figure
4
), note that the 445 nm peak, occurring in all but an empty well, is the Raman
spectrum for water. Note that the optimal excitation wavelength is 485 nm.
Scan Figure 4
5 Comments
a
In endpoint or kinetic fluorescence modes, the Autofilter feature will generally select
the same cutoff filter wavelength as will the above optimization method. If desired,
however, you may specify the cutoff filters manually.
b
For emission wavelengths where there is no cutoff filter (less than 420 nm or greater
than 695 nm) experimental iteration is usually the best method of determining the
optimal emission and excitation wavelengths. Begin optimization by performing steps
1–3 above. For excitation wavelengths greater than 695 nm, use the 695 emission
cutoff filter. Note that the emission optics block all wavelengths below 360 nm.
84
FlexStation II Operator’s Manual – Rev. D
Summary of Contents for FlexStation II
Page 6: ...FlexStation II Operator s Manual Rev D vi ...
Page 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Page 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Page 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Page 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
