3 Operating Procedures
3.5.9 Compound
Transfer
Compound Transfer is an important setting to configure correctly. In addition to
configuring precise fluid transfers for your experiments, this dialog box also helps
prevent flooding of the assay plate.
The fields in this dialog box will allow you to set volumes for up to three transfers.
However, you must be careful to keep in mind the actual maximum volume allowed in
the wells you are using as you move through the settings in this dialog box. The
maximum cumulative volume depends on the assay plate type you select.
Compound Transfer Dialog Window
Assay Plate Fluid/Initial Volume
Enter a value in the Assay Plate Fluid box that equals the largest initial volume (prior to
compound transfers) for any well in the assay plate. This value can be set at 0–
269 microliters (µL), although obviously typical values will be about 10–200 µL for a
96-well microplate.
SOFTmax PRO assumes all wells hold the same initial volume. The software uses this
value to compute the total volume in each well after all fluids have been dispensed. The
software makes this calculation to warn you regarding potential overflow of fluid from
the wells.
If there is no fluid volume in the assay plate prior to compound transfers, do not enter a
value in this box.
Any value entered is saved with the file but the value is not displayed anywhere except in
the Compound Transfer setting.
Values for a 384-well microplate can be set at 0–120 µL.
64
FlexStation II Operator’s Manual – Rev. D
Summary of Contents for FlexStation II
Page 6: ...FlexStation II Operator s Manual Rev D vi ...
Page 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Page 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Page 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Page 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
