3 Operating Procedures
to select a cutoff filter at, or even above, the lambda maximum. For example, this is a
good idea with fluorophores with small Stokes shift or when multiple fluorophores must
be discriminated. For further information, see section 3.10, Optimizing Fluorescence
Assays in Spectrum Mode.
Important:
If you select an emission wavelength that is close to two times the excitation
wavelength (if EM
λ
= 2 × EX
λ
) always be sure to use an emission cutoff filter to ensure
you are blocking monochromator second order light. If you run an experiment like this
without an emission filter, you will always observe an emission peak at two times the
excitation wavelength, but it is not fluorescence emission.
56
FlexStation II Operator’s Manual – Rev. D
Summary of Contents for FlexStation II
Page 6: ...FlexStation II Operator s Manual Rev D vi ...
Page 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Page 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Page 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Page 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
