Molecular Devices FlexStation II, Operator'S Manual

Page: 126 / 134

7 Appendices
7.2 Performance Specifications
Fluorescence Photometric
Excitation Wavelength Range 250–850 nm
Emission Wavelength Range 360–850 nm
Excitation Wavelength Bandwidth 9 nm
Emission Wavelength Bandwidth 18 nm
Wavelength Selection Scanning monochromator
tunable in 1-nm increments
Wavelength Accuracy ± 2 nm
Sensitivity (signal 3×SD of baseline)
96 wells, Top Read
3 fmol/well FITC
96 wells, Bottom Read 8 fmol/well FITC
384 wells, Top Read 3 fmol/well FITC
384 wells, Bottom Read 6 fmol/well FITC
Time-Resolved Fluorescence, 384 wells 0.5 fmol/well Eu-Ch
Calibration Self-calibrating with built-in
fluorescence calibrators
Luminescence Photometric
Sensitivity 10 amol ALKPhos
Wavelength Range 360–850 nm
General Photometric
Microplate Formats 6, 12, 24, 48, 96, 384
Light Source Xenon flash lamp (1 joule/flash),
user-replaceable
Lamp Life Two years normal operation,
one-year warranty
Detector Photomultiplier (R-3896)
Read Time < 9 sec (96 wells), < 29 sec (384 wells)
(measurement type may extend read time)
Single-Column Read Frequency, 96-wells 1 Hz
Single-Well Read Time Frequency 20 Hz
Detection Spot Size, Top Read 3.5 mm
Detection Spot Size, Bottom Read 1.5 mm
Shaker Time 0–999 seconds
Temperature control (reading chamber) From 1
°
C above ambient to 37
°
C in 30 min
SOFTmax PRO Software Windows 95/98/NT/2000 compliant
System Validation Internal standards for fluorescence and
wavelength
Fluidics, 8-channel pipettor
Volume Range 1–200 µL
Dispensing Precision 8% CV at 5 µL, 2% CV at 50 µL
Maximum Dispense Rate 208 µL/sec
Fluidics, 16-channel pipettor
Volume Range 1–30 µL
Dispensing Precision 3% CV at 10 µL, 5% CV at 1 µL
Maximum Dispense Rate 52 µL/sec
120 FlexStation II Operator’s Manual – Rev. D