3 Operating Procedures
•
Time Point
(Minimum Time to 9999 seconds): This setting determines the time
after the start of the reading when the fluid is scheduled to begin being dispensed.
Your time point will eventually be your baseline time. (This is not the time interval
between transfers.)
Important!
The Time Point cannot be smaller than the Minimum Time identified by
the software for each transfer.
•
Minimum Time:
This information line automatically displays the minimum time
required before a pipetting event can occur. This minimum time value is cumulative
(not an interval between pipetting events). The value is the minimum number of
seconds of elapsed time from the beginning of the read. It takes into account the
mechanical speed of the pipette head and the time needed to aspirate and dispense
fluids, trituration, and Automixing.
The minimum time for the second pipetting event depends on when the first
pipetting event occurred. The calculation for the second event starts at the end of the
first event and adds to that the total time that will be necessary to aspirate new fluids
from the compound plate and dispense them into the assay plate.
Possible Problems
Time point calculations are based on the number of wells read, the tip column and
compound column used during transfer. If you select time points that are not long
enough (incompatible with the selected volumes and transfer speed) you will see one
or both of the following messages:
If you select transfer volumes that add up to more than the maximum can be
accommodated by the assay plate, this overflow warning will appear:
Be sure to pay attention to the minimum time value displayed under the time point
box for each transfer, as the minimum time values are different depending on which
transfer you are configuring.
Note:
The Compound Transfer setting works in conjunction with the Compound & Tip
Columns setting described below. Until well assignments are made in the
Compounds & Columns setting, the Compound Transfer setting will show ‘No targets
assigned.’
66
FlexStation II Operator’s Manual – Rev. D
Summary of Contents for FlexStation II
Page 6: ...FlexStation II Operator s Manual Rev D vi ...
Page 92: ...4 Applications 86 FlexStation II Operator s Manual Rev D ...
Page 102: ...5 Service and Maintenance Procedures 96 FlexStation II Operator s Manual Rev D ...
Page 124: ...6 Troubleshooting Procedures 118 FlexStation II Operator s Manual Rev D ...
Page 134: ...7 Appendices 128 FlexStation II Operator s Manual Rev D ...
