MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 92 / 157

7-2
DNA Engine Dyad Operations Manual
In Chapter 5, we translated an experimental protocol into functional Dyad programs. In
Chapter 6, we edited program steps. In this chapter, we explore the implementation of the
Dyad programs, and the instant incubation feature.
Using the Instant Incubation Feature
The DNA Engine Dyad cycler can also be quite useful as an “instant” constant-temperature
incubator, with a range from –5.0
°
C to 105
°
C (4
°
C to 100
°
C with the Twin Towers
®
block).
This feature can be used for performing ligations, digestions, etc.—or with slides, overnight,
humidified hybridizations.
Note: The Twin Towers block can be used as a humidified chamber for steady-state incuba-
tions (e.g., hybridizations, color-development reactions). To humidify a block, push one labo-
ratory tissue into the bottom slot and inject 1ml of deionized water onto it. See the
Twin
Towers Operations Manual for complete instructions.
To initiate an instant incubation from the Dyad Status window,
• Select <Instant> at the bottom of the window or from the <Com-
mand> menu.