MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 61 / 157

5-23
Creating Programs
Step one of our protocol now consists of a temperature step with an incubation tempera-
ture of 94.0 and a duration of 01:00.
Recall that the maximum programmable temperature is 105.0
°
C and the minimum pro-
grammable temperature is 0.0
°
C in a graphical program. The maximum duration of a
temperature step in a graphical program is 99 minutes and 59 seconds or forever.
Step two of our program is also a temperature step, but with an incubation temperature
of 92.0
°
C and a duration of 30 seconds.
If step two in the displayed protocol is a temperature step, follow the instructions in
scenario 3 and in the “Editing Step Parameters” section to alter the parameters of the
displayed step.
If step two is not a temperature step:
• Select step 1.
• Select <Temp>.
A new temperature step will be added after step 1 with the default temperature and time
parameters. Follow the instructions in scenario 3 and in the “Editing Step Parameters”
section above for information on altering the step parameters.
Step 1
temperature step