MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 59 / 157

5-21
Creating Programs
Entering a Temperature Step
Recall again our raw program:
Use calculated temperature control mode
Use constant lid control mode at 100
°
C
An initial incubation at 94
°
C for 1 minute
1. 92
°
C for 30 seconds
2. Gradient from 45
°
C to 65
°
C for 3 minutes
3. Goto step 1, 29 more times
4. An incubation at 10
°
C forever
5. END
The first actual step in the protocol is the incubation at 94
°
C for 1 minute. There are three
scenarios for programming this initial temperature step.
1. If there are no steps displayed:
• Select <Temp>.
A temperature step will be added as the first step in the protocol with a default
temperature of 55.0
°
C and a duration of 30 seconds.
Proceed to scenario 3 for instructions on editing step parameters.
2. If the first step displayed is a gradient step:
• Select the gradient step (step 1).
• Select <Temp>.
This will add a new temperature step to the protocol as step 2 with a default
temperature of 55.0
°
C and a duration of 30 seconds.
Note: Steps are always added after the step that is currently selected.
• Select the gradient step (step 1).
• Select <Delete Step>.
The initial gradient step will be deleted, and the newly added temperature step
will be promoted to step one.
Proceed to scenario 3 for instructions on editing step parameters.