MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 58 / 157

DNA Engine Dyad Operations Manual
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Selecting a Step
When a step is selected, the line(s) or arrow depicting that step will turn red. There
are several ways to select a step. You can select a step by positioning the cursor in
the step’s column and tapping or clicking once. A flashing insertion point will appear
in the temperature field of that step. To select a specific temperature or time field (or
a goto step), position the cursor, its shape will change to a text pointer, in the desired
field and tap/click once to display an insertion point, or tap/click twice to highlight
the entire field. Alternatively, use the left/right cursor keys to sequentially select the
temperature, time, and/or number of cycles fields for the program steps.
Editing Step Parameters
If you have selected a step such that a flashing insertion point appears in the field you
wish to edit, use the backspace and number keys on the numeric keypad to first
delete the current value, then enter the desired temperature, time, or number of cycles.
Tap the touch pad or click the mouse once to accept the change, or press the left/
right cursor key once to accept the change and move to the next field. If you have
selected a step such that the field you wish to edit is highlighted, use the number keys
to enter the desired temperature, time, or number of cycles. Tap the touch pad or click
the mouse once to accept the changes.
If an inappropriate value is entered, such as an incubation temperature of 110
°
C,
the change will be rejected, and the default value or last valid value will reappear.
Deleting a Step
To delete a step, first select the step as indicated above such that the line or arrow
depicting that step turns red. Then, select the <Delete Step> button. The selected step
will be deleted and the following step will automatically be promoted.
Adding a Step
In graphical programs, a step is added directly after the step that is currently se-
lected. Graphical programs can contain a total of six temperature and gradient steps
and one goto step. To add a step, select the step that will immediately proceed the
new step. Then, select either the <Temp>, <Grad>, or <GoTo> button to add either a
temperature, gradient or a goto step to the program. A goto step can not be the first
or only step in a program. See the sections immediately following for complete in-
structions on adding specific types of steps and entering step parameters.
Now, let’s begin entering the steps for our example program.