MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 44 / 157

DNA Engine Dyad Operations Manual
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Types of Programs
There are two types of Dyad programs, graphical and advanced. Graphical programming
features a graphical interface and a graphical representation of the program steps. Ad-
vanced programming features a text-based interface and a descriptive listing of the program
steps.
Graphical Programs
Creating a graphical program is desirable if you prefer the graphical programming interface
and/or wish to quickly enter a program that:
• Does not contain more than a total of six temperature and/or gradient steps
• Does not contain temperature or gradient steps that contain modifications (i.e., increment
temp, extend time, slow ramp, beep)
• Does not contain more than one goto step
• Does not contain a temperature incubation below 0
°
C
• Does not contain a step with an incubation lasting more than 1 hour 39 minutes and 59
seconds (99:59). Forever incubations excepted.
In addition to the speed with which they can be entered, another benefit of graphical pro-
grams is that they can be quickly edited. This can be particularly useful if you tend to repeat-
edly run the same general protocol with limited changes (e.g., varying annealing tempera-
tures).
To create a graphical program, refer to the “Entering a Program Using Graphical Mode”
section below.
Graphical program Advanced program