MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 70 / 157

DNA Engine Dyad Operations Manual
5-32
• Select <Temp>.
• Position the cursor over the
Temperature field and select.
• Enter 92 from the numeric keypad into the field.
• Similarly, enter 30 in the Time:Sec field.
Additionally, we want the cycler to beep after reaching the target temperature.
• Select Beep on Target.
At this point, in the Temperature Step window, all selections have been made
according to our protocol.
• Select <OK>.
In the advanced programming window, the program listing now appears as
follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100
°
C
1. Incubate at 94
°
C for 00:01:00
Beep on Target.
2. Incubate at 92
°
C for 00:00:30
Beep on Target
End
The 92
°
C denaturation is the first step in the cycling portion of our program. The
next step is the gradient step.
• Highlight Step 2 in the program listing.
• Select <Gradient>.