MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 69 / 157

5-31
Creating Programs
The Temperature Step window presents the operator with a number of tempera-
ture adjustment options. The
Temperature and Time fields as well as the Beep on
Target selection option are available at the top of the window. At the bottom, the
Increment Temp, Extend Time and Slow Ramp options are available, each with
their own <Set Parameters> button. For this particular step, the only parameters
that will be set will be the temperature and the incubation time.
Note: The Alpha units can ramp at a maximum of 3.0
°
C per second. If a slower
ramp speed is not entered, this will be the default.
• Position the cursor over the Temperature field and select.
• Enter 94 from the numeric keypad into the field.
• Similarly, enter 1 in the Time:Min field.
Additionally, we want the cycler to beep after reaching the target temperature.
• Select Beep on Target.
At this point, in the Temperature Step window, all selections have been made
according to our protocol.
• Select <OK>.
In the advanced programming window, the program listing now appears as
follows:
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100
°
C
1. Incubate at 94
°
C for 00:01:00;
Beep on Target.
End
The program, if run now, would ramp to 94
°
C for 1 minute and then end. Let’s
continue to enter steps and build our program.
The first temperature step in the cycling portion of our program incubates at 92
°
C
for 30 seconds. We would also like the cycler to beep after reaching this tem-
perature.
• Position the cursor over the appropriate insertion point, if not high-
lighted already, and select.
Note: The appropriate insertion point would be selected by highlighting the first
step. Insertion will occur AFTER this step.