MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 72 / 157

DNA Engine Dyad Operations Manual
5-34
The newly created gradient can be previewed graphically.
• Select <Preview>.
This distribution of temperatures specified by the gradient should be reviewed
and any changes to the gradient limits made before a program run. Please note
that the gradient temperature differential is not linear, with a broader spread in
temperature between the center columns of wells. This is a consequence of the
geometry of the Peltier-Joule heaters that underlie the block and is normal. Rest
assured that the temperatures displayed are quite accurate for each well in that
column (
±
0.4
°
C of actual column temperature).
• Select <Close>.
• Select <OK>.
The program listing should now appear as follows. Since the program step listing
field is limited, please use the directional arrow keys to navigate through the
steps to review them.
Temperature Control Mode: Calculated
Lid Control Mode: Constant at 100
°
C
1. Incubate at 94
°
C for 00:01:00
Beep on Target
2. Incubate at 92
°
C for 00:00:30
Beep on Target
3. Gradient from 45
°
C to 65
°
C for 00:03:00
End