MJ Research DNA Engine Dyad PTC-220, Operation Manual, Page: 21 / 157

Operation
4
Turning the DNA Engine Dyad Cycler On, 4-2
Using the Control Panel, 4-3
Display Screen, 4-3
Operation Keys, 4-3
Block Status Lights, 4-4
Using the Data Ports, 4-4
Operating Alpha Units, 4-4
Installing an Alpha Unit, 4-4
Removing an Alpha Unit, 4-5
Opening an Alpha Unit, 4-6
Closing an Alpha Unit, 4-6
Selecting the Correct Sample Vessel, 4-6
0.5ml Tubes, 4-7
Thin-Walled vs. Thick-Walled Tubes, 4-7
0.2ml Tubes, 4-7
Microplates, 4-7
Sealing Sample Vessels, 4-8
Sealing with Oil or Wax, 4-8
Sealing with the Hot Bonnet Lid, 4-8
Adjusting the Hot Bonnet Lid’s Pressure, 4-9
Loading Sample Vessels into the Block, 4-10
Using Oil to Thermally Couple Sample Vessels to the Block, 4-10
Using the Optional Probe, 4-11
Customizing the Probe Vessel, 4-11
Adding the Oil, 4-12
Loading and Connecting the Probe, 4-12
Detecting a Faulty Probe, 4-13
Appendix 4-A: Tube, Microplate, and Sealing System Selection Chart, 4-14
Appendix 4-B: Safety Warning Regarding Use Of
35
S Nucleotides, 4-15
Appendix 4-C: Using Silicone Oil in the Probe Tube, 4-17