Milestone s.r.l.
MM098-002
– LOGOS ONE – Operator Manual
125
A.2. OVERVIEW OF HISTOPROCESSING TROUBLE SHOOTING
Problem
Cause
Remedy
Pyknotic
nuclei
and
severe
cytoplasmic
distorsion
Poorly fixed.
Extend fixation time.
Poor fixation/JFC reaction.
Use JFC/Isopropanol program.
Poor Dehydration
Alcohol contamination (water).
Replace alcohol.
Use of sponge Pads/ inefficient biopsy Pads.
Extend processing time.
Use sponge alternatives.
Processing cycle too short.
Use appropriate size processing cycle.
Specimens detached from surrounding WAX
Processing cycle too short.
Use appropriate size processing cycle.
Reduce tissue block thickness.
Incomplete Clearing of fat
Processing cycle too short.
Use appropriate size processing cycle.
Reduce tissue block thickness.
Isopropanol inefficient for fat clearing.
Use JFC as alternate clearing agent.
Reduce tissue block thickness.
Dehydration/Clearing agent reuse life exceeded.
Replace Dehydration or Clearing reagent.
Collapse of block face after sectioning
WAX infiltration not entirely complete.
Increase WAX infiltration time.
White appearance of block face
WAX infiltration not entirely complete.
Increase WAX infiltration time.
Pigment deposit in sections
Incomplete fixation of blood.
Perform pigment test to exclude whether formalin
pigment or hemosiderin deposits.
Include heated fixation or extend conventional fixation
time.
Fatty tissue collapse in blocks & microsections
Inadequate fixation of tissue, reacting with JFC.
Fix tissue conventionally overnight or include heated
fixation.
Use JFC followed by Isopropanol protocol for processing.
Absence of connective tissue parenchyma to
support delicate fatty network structure.
Use JFC followed by Isopropanol protocol for processing.