Milestone s.r.l.
MM098-002
– LOGOS ONE – Operator Manual
120
PROBLEM
BLOCK/SLIDE
POSSIBLE CAUSES
RIMEDIAL ACTION
Inadequate Clearing of
fat.
The
block face
has
a
“greasy” feeling.
Tissue
block
difficult
to
section.
When section placed on
water
bath
the
section
breaks up.
Softness of some areas of
the block after the trimming.
1.
Processing
time
for
tissue
is
inadequate for the size selected,
resulting in incomplete removal of fats,
especially from fatty specimen blocks,
such as breast. This can be seen when
processing the larger blocks, where
processing times become more critical
to accommodate the larger sizes.
2.
Use of isopropanol as clearing agent
for fatty tissues is inefficient for the
removal of fats, especially the larger
blocks of breast.
3.
Reuse life of dehydration or clearing
reagent has been exceeded.
1.
Select the correct processing time, or select
longer processing time for the sample size.
AND
Reduce tissue thickness when preparing wet
tissue blocks, if reducing processing times is
not desired.
2.
Use JFC as the alternative clearing agent.
AND
Significantly reduce tissue block thickness
(50%) as well as increase WAX impregnation
time (compromise method, not recommended
as it may be impractical to implement
routinely).
3.
Replace the dehydration/clearing reagent or
reduce the number of times the solution is
reused.