Labconco Logic+ A2, Technical Manual

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Type C1 Exhaust HEPA Filter Leak Test Preparation
All biosafety cabinet models were tested to the HEPA Filter Leak Test as described in
the current NSF/ANSI Standard 49. This section describes the methods, specifications,
and Pass/Fail criteria to challenge the exhaust HEPA filter in the biosafety cabinet.
You never know what a biosafety cabinet has been exposed to, so it is always
recommended to surface decontaminate any components prior to working inside the
biosafety cabinet and/or removing components from the biosafety cabinet.
1. Remove the center work surface by lifting the handles on either end. Pull the
work surface straight out of the biosafety cabinet. Remove the two work surface
wings.
2. Turn the blower of the biosafety cabinet on.
3. Place the aerosol generator on the center drip tray exposed when the center
work surface was removed, see Figure 10-22.
4. Use an extension tube (see Figure 10-22) on the aerosol generator to ensure the
challenge aerosol is delivered to the center part of the rear baffle (defined by the
center drip tray the generator is resting on). The challenge must not be delivered
to the far left or right portion of the rear baffle.
5. Check the oil level of the aerosol generator is within 1/8 inch (3 mm) of the level
line.
6. If the aerosol generator requires pressurized air, connect the air line.
7.
Verify the generator’s air pressure is 23 psi.
8. Turn on the photometer and allow it to operate for a minimum of 5 minutes.
Leave the valve in the “CLEAR” setting.
Theoretical Upstream Concentration
Actual upstream sampling is not possible on a Type C1 exhaust filter, so the theoretical
concentration will be used. Testing at Labconco has shown the actual concentration
varies from the theoretical value. Table 10-5 shows the actual vs. calculated
concentrations for Type C1 models. Establish the 100% and 0% concentration levels for
the photometer using the theoretical values provided in Table 10-5 for the model being
tested.