Hologic ThinPrep 5000, Operator'S Manual

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ThinPrep™ 5000 System Instructions for Use English AW-22289-001 Rev. 003 11-2021 3/36
The ThinPrep Sample Preparation Process
(1) Dispersion (2) Cell Collection (3) Cell Transfer
The sample vial is rotated,
creating currents in the fluid that
are strong enough to separate
debris and disperse mucus, but
gentle enough to have no
adverse effect on cell
appearance.
A gentle vacuum is created within
the ThinPrep Pap Test Filter, which
collects cells on the exterior
surface of the membrane. Cell
collection is controlled by the
ThinPrep 5000 Processor’s
software that monitors the rate of
flow through the ThinPrep Pap Test
Filter.
After the cells are collected on
the membrane, the ThinPrep Pap
Test Filter is inverted and gently
pressed against the ThinPrep
Microscope Slide. Natural
attraction and slight positive air
pressure cause the cells to
adhere to the ThinPrep
Microscope Slide resulting in an
even distribution of cells in a
defined circular area.
As with conventional Pap smears, slides prepared with the ThinPrep™ 5000 System are examined
in the context of the patient’s clinical history and information provided by other diagnostic
procedures such as colposcopy, biopsy, and human papillomavirus (HPV) testing, to determine
patient management.
The PreservCyt™ Solution component of the ThinPrep 5000 System is an alternative collection
and transport medium for gynecologic specimens tested with the Digene Hybrid Capture

System HPV DNA and Hologic APTIMA COMBO 2™ CT/NG Assays. Refer to the respective
manufacturer’s package inserts for instructions for using PreservCyt Solution for collection,
transport, storage, and preparation of specimens for use in those systems.
The PreservCyt Solution component of the ThinPrep 5000 System is also an alternative
collection and transport medium for gynecologic specimens tested with the Roche Diagnostics
COBAS AMPLICOR
TM
CT/NG assay. Refer to Hologic’s labeling (Document #MAN-02063-001) for
instructions for using PreservCyt Solution for collection, transport, storage, and preparation of