Hologic ThinPrep 5000, Operator'S Manual

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N
ON
-G
YNECOLOGIC
S
AMPLE
P
REPARATION
5.18
ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5
URINE SPECIMENS FOR THE VYSIS
®
UROVYSION ASSAY
For use with Vysis UroVysion. If performing urine cytology, follow the Body Fluids protocol.
7. Allow to stand in PreservCyt Solution for 15 minutes (page 5.11)
8. Run on ThinPrep
™
5000 processor with AutoLoader using
Sequence
Non-Gyn. Fix, stain, and evaluate.
1. Collection. Collect urine, OR process urine fresh.
Note:
Fresh urine can be mixed with a 2:1 urine-to-PreservCyt
™
Solution ratio and stored for up to 48 hours before processing.
Note:
Do not exceed a 2:1 ratio of urine to PreservCyt
™
Solution. If the
urine volume exceeds 60 mL, pour off excess. A minimum
volume of 33 mL of urine is required to perform the Vysis
®
UroVysion assay.
2. Concentrate by centrifugation (page 5.5)
Transfer the sample evenly into two labeled 50-mL centrifuge tubes.
Centrifuge at 600g for 10 minutes or 1200 g for 5 minutes.
3. Pour off supernatant and resuspend cell pellet (page 5.7)
Resuspension can be done on a vortexor or may be achieved by syringing
the pellet back and forth with a plastic pipette.