Hologic ThinPrep 5000, Operator'S Manual

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ThinPrep™ 5000 Processor with AutoLoader Operator’s Manual
5.1
5
N
ON
-G
YNECOLOGIC
S
AMPLE
P
REPARATION
C h a p t e r F i v e
Non-Gynecologic Sample Preparation
INTRODUCTION
This chapter provides instructions for preparing non-gynecologic (non-gyn) samples and making
slides with the ThinPrep
™
5000 system.
For the best results, carefully follow the instructions in this chapter. Because there is biological
variability among samples and variability in collection methods, standard processing may not
always yield a satisfactory and uniformly distributed preparation on the first slide. This chapter
contains troubleshooting instructions for further sample processing to obtain better quality
subsequent slides in these cases. This chapter also provides an outline of various sample collection
methods and the appropriate procedures for each.
Content found in this chapter:
REQUIRED MATERIALS
SPECIMEN COLLECTION
METHODS OF SAMPLE PREPARATION
• Concentrate by centrifugation — 600g for 10 min.
• Pour off supernatant and vortex to resuspend cell pellet
• Evaluate cell pellet appearance
• Add specimen to PreservCyt
™
Solution vial
• Allow to stand in PreservCyt Solution for 15 min.
• Run on ThinPrep
™
5000 processor with AutoLoader using Sequence Non-Gyn. Fix,
stain, and evaluate.
• Mechanical agitation
• CytoLyt
™
Solution wash
SPECIMEN PREPARATION GUIDELINES
• Fine Needle Aspirates
• Mucoid Specimens
• Body Fluids
• Urine Specimens for the Vysis
®
UroVysion assay
SAMPLE PREPARATION TROUBLESHOOTING
SECTION
A