3.2 Automatic Continuous Measurement (6-cell auto mode)
3 - 20
(cont’d)
In the case where a sample contains an interfering substance such as turbid or disturbing
component as illustrated at <6> in the above figure, the absorbance due to the interfering substance
will be added to the quantitative value of the analyte. In this case, it is necessary to carry out a
blank test (the same preparatory operation as for samples by use of pure water or the like) and
subtract the measured absorbance of the blank test solution by auto-zero operation at the time of
sample measurement or subtract the quantitative value of the blank test solution from that of the
sample in order to subtract the absorbance of the interfering substance from that of the sample.
For quantitation of the sample with 6 Cell Mode set to Auto and subtraction by auto zero, the STD
Autozero, SAMP. Autozero and Thru Zero are set to STD1, Sample Blank and ON, respectively.
(Refer to the measurement parameters in “F” of the following table.)
Setting of Each Measurement Parameter with Coloring Reagent
D: Usual Method
(in case of
sample <4>)
E: Auto Zero with
Blank (in case of
sample <4>)
F: Correction with Blank
Test Solution (in case of
sample <6>)
Application
Common Usage
(auto zero with
STD1)
When STD1
Absorbance is
Unstable with Time
(auto zero with
blank)
For Subtracting Absorbance
of Blank Test Solution
STD Autozero
STD1
Blank
STD1
SAMP. Autozero ON
OFF
ON
OFF
Sample Blank
Thru Zero
ON
OFF
ON
