Hach DR/850, Procedures Manual, Page: 275 / 460

275
Summary of Method
Ammonia compounds combine with chlorine to form
monochloramine. Monochloramine reacts with salicylate to form 5-
aminosalicylate. The 5-aminosalicylate is oxidized in the presence of a
sodium nitroprusside catalyst to form a blue-colored compound. The
blue color is masked by the yellow color from the excess reagent
present to give a green-colored solution.
Safety
Good safety habits and laboratory techniques should be used
throughout the procedure. Consult the
Material Safety Data
Sheets for information specific to the reagents used. For
additional information, refer to Section 3 .
Pollution Prevention And Waste Management
The ammonia salicylate reagent contains sodium
nitroferricyanide. Cyanide solutions are regulated as hazardous
wastes by the Federal RCRA. Collect cyanide solutions for
disposal as reactive (D001) waste. Be sure cyanide solutions are
stored in a caustic solution with pH >11 to prevent release of
hydrogen cyanide gas. See
Section 3 for further information in
proper disposal of these materials.
REQUIRED REAGENTS
AmVer
™
Reagent Set for Nitrogen, Ammonia, High Range, TNT (25 tests) ..............26069-45
Includes: (1) 23952-66, (1) 23954-66, (1) 272-42,
*
(50) AmVer HR Vials
Quantity Required
Description Per Test Unit Cat. No.
AmVer
™
HR Reagent Test ‘N Tube
™
Vials ............ 2 vials ...................50/pkg ....................... *
Ammonia Salicylate Reagent Powder Pillows....... 2 pillows.................50/pkg ..........23952-66
Ammonia Cyanurate Reagent Powder Pillows ...... 2 pillows.................50/pkg ..........23954-66
REQUIRED APPARATUS
COD/TNT Adapter........................................................... 1 .................each .................48464-00
Pipet, TenSette
®
, 0-1 mL ................................................. 1 .................each .................19700-01
Pipet Tips for 19700-01................................................ varies ......... 50/pkg .................21856-96
Test Tube Rack ............................................................... 1-3 ...............each .................18641-00
Funnel, micro (for reagent addition) ................................ 1 .................each .................25843-35
* Not available separately.
NITROGEN, AMMONIA, High Range, Test 'N Tube,
continued