Biometra TOne
Operation
47
Program parameters
Display
(example)
Description
Heat.-/Cool rate
4.0
Between the steps, the device will heat or
cool the sample block by the specified aver-
age heating or cooling rate [°C/s].
Gradient
55.0
+1.0
(at
Block Temp.
(°C)
)
The block temperature increases or de-
creases column by column by the tempera-
ture increment [°C] starting from the anneal-
ing temperature [°C].
Understanding the gradient
view
During each gradient step, the gradient view displays the temperatures that are applied
in each of the columns of the sample block.
55.0
61.0
60.0
59.0
58.0
57.0
56.0
66.0
65.0
64.0
63.0
62.0
1
12
11
10
9
8
7
6
5
4
3
2
°C
Column
Fig. 25
Gradient view (during gradient step, block format 96-well)
5.8.2
Pausing or stopping executed programs, skipping steps
You have the option to pause, resume or stop the program during a PCR run. You can
also skip individual steps.
Option
Icon
Description
Pause
Abort the program at the current step
The graphical and spreadsheet view highlights the cur-
rent step in blue.
The field
Step
in the table in the header reads
Pause
.
The system counts the duration of the pause. The in-
formation displayed in the field
Estimated End
is
adapted accordingly.
Continue
Resume program after a pause
Skip
Skip the current step. Continue the program with the
subsequent step
Stop program
Stop program
An additional prompt to confirm this selection helps
avoiding operating errors.
If you switch off the device during a pause step, this will be recognized as a power failure
by the software. The software will generate a corresponding error message and record it
in the run log file. Once the device is restarted, the software will resume the program.
