Biometra TOne
Operation
37
Temperature:
Hold Time:
Go To:
Cycles:
Grad
Step
GoTo
30x Step
2
Fig. 15
Defining all parameters for a step
}
For gradient-enabled models, define a gradient as required:
– Tap on the block temperature field in the graphical or spreadsheet view. Tap on
the button
Degrees
on the numeric keypad.
– Or: Tap on the button
Degrees
on the extra page for programming all parame-
ters.
}
Save the new or edited program after changing all required parameters.
ü
You have edited one or several program steps.
Inserting steps
}
Use the
Insert
button to insert additional steps in the program. The maximum num-
ber of steps in a program is 30.
ü
The new step will be inserted at the selected program step. This will not over-
write the existing step. Instead, this step and any subsequent steps will by moved
down one position.
Deleting steps
}
Use the button
Delete
to delete individual steps.
ü
The selected step is deleted. All subsequent steps will move up one position.
5.6.6
Programming a loop
A typical PCR program consists of cyclically reiterated steps for:
¡
Denaturation
¡
Annealing
¡
Extension
To reiterate steps, create program loops:
ð
Prerequisite: You have opened an existing program or a program template for edit-
ing.
}
Tap the button
GoTo
in the graphical view. Tap on the field for the step in the line
underneath the diagram with which you want the loop to begin.
}
Or: Tap on the field of the step with which you want the loop to begin in the column
GoTo
or
Cycles
in the spreadsheet.
}
Or: Select the step. Tap
Edit
to open the extra page for programming all parameters.
