Biometra TOne
Operation
31
}
Use the input field
Lid Temperature:
to set the temperature for the heated lid. You
also have the option to enable the preheating function for the heated lid by tapping
on the button
On
.
}
You can use the buttons
Insert
and
Delete
to insert additional steps or to delete ex-
isting steps in the program. The maximum number of steps in a program is 30.
}
Edit the individual program steps. To do this, tap on a program step to select it. The
selected program step is highlighted in blue.
– Edit the individual parameters for this step directly in the spreadsheet or diagram.
– Or: After tapping
Edit
change all parameters on a particular page.
}
Define at least one block temperature (3 to 99 °C) and holding time (max.
≤9 h 59 min 59 s) for each program step. Enter
∞
to program a pause of infinite du-
ration.
}
Define the following optional parameters:
– Program loops
Program loops are repeated in cycles. The maximum number of iterations is 999.
Define the step number to which the program shall return under
GoTo
. Define
the number of repeated cycles under
Cycles
.
– Temperature increment
ΔT
The block temperature increases or decreases by the temperature increment with
each cycle. The temperature increment can be ±0.1 to 20 °C.
– Time increment
Δt
The holding time increases, e.g. in case of a long-range PCR, by this time incre-
ment with each cycle. The time increment can be 1 to 240 s.
– Heating and cooling rate
ΔR
The block temperature is controlled by the specified heating or cooling rate. The
value can be set by increments of 0.1 °C/s. The maximum heating and cooling
rate depends on the device model.
– Temperature gradient
Degrees
(only for gradient-enabled models)
The block temperature increases or decreases by a specified temperature gradient
from column to column in the sample block. The maximum temperature range
depends on the device model.
}
Once all program steps are completed, tap on
Done
.
}
Select a user directory and a storage location for the program on the page
Copy/save
programs
.
}
Tap
Save
to save the program.
}
Or: Tap
Save as
to save the program under a new name. The system will overwrite
the initial name.
ü
You have created a program for a PCR run. The program execution time is shown
under
Program information
in the preview.
You can now tap
Start
to start the program.
After this brief instruction, we will now proceed with a comprehensive description of the
individual program parameters including important tips and information for the pro-
gramming process.
