Chapter 3 Scanning Operations
3-16
Applied Biosystems
3
3.3 Setting Up Multiple Scans in a
Cycle
A single cycle can consist of one to four scans. Each scan is a
separate pass of one microtiter plate. The scans can have
different excitation and emission filter pairs and/or gains, or
they can be identical.
To set up multiple scans in a cycle, you need to select the:
•
Number of scans per cycle (one to four)
•
Number of the scan you want to define
•
Filter pairs for each scan
•
Gain for each scan
•
Set additonal scans, and copy settings, if needed
•
Save the file
NOTE: You need to access the CytoFluor main setup
screen to select the multiple scan settings. See the
following sections for details.
Select the number
of scans
1.
Select the number of scans you want in each cycle
from the Scan/cycle box if you have not done so
already. See “Scans/Cycle” on page 3-11 for details.
2.
Click the down arrow in the Scan field to reveal a
pull-down list.
3.
Select the scan you want to define (1, 2, 3, or 4).
4.
Select the filter pair level for the scan as described in the
next section.
Select the filter
pairs for a Scan
1.
Click on the down arrow in the Excitation field to
display the list of choices.
2.
Highlight the filter pair you want to use.
3.
Click on the down arrow in the Emission field to display
the list of choices.
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