Using Well Types Options
CytoFluor User’s Guide
5-13
5
Empty
Use this option to define any wells on your assay plate that
have been left unfilled or ones you do not want considered in
the assay.
NOTE: The system does not treat Empty wells the same
way it treats Blank wells. It uses Blank wells to calculate a
background value to subtract from all wells. The system
collects scanned data of Empty wells, but does not use their
values in calculations.
To define wells as Empty:
1.
Click on the Empty button in the Plate Layout screen.
2.
Select the well(s) on your assay plate you want as empty.
(Click with your mouse, or click and drag to select a group
of wells.) The system enters the abbreviation “emp” into
the wells you select.
3.
Make additional changes to the plate layout as described
in this chapter. Or click on OK to save your changes.
Hidden
This well option enables you to omit outlying data from
calculations. The data is not lost, but omitted from any
calculations. For example, values that exceed the system
limits (where +++) appears as the maximum value).
To define wells as Hidden:
1.
Click on the Hidden button in the Plate Layout screen.
2.
Select the well(s) on your assay plate you want as hidden.
(Click with your mouse, or click and drag to select a group
of wells.) The system enters the word “hide” in the wells
you select.
3.
Make additional changes to the plate layout as described
in this chapter. Or click on OK to save your changes.
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