Setting Scan Parameters
CytoFluor User’s Guide
3-15
3
Total
Time/Elapsed
Before a run, displays the total run time. During the run,
displays the time elapsed between the current scan and the
same scan number acquired during the first cycle. You cannot
edit this field. During a run, this field doubles as a countdown
timer to the start of the next cycle.
Auto Scale
Settings
Enables you to see the auto scale selections in effect for the
run if the Auto scale box is selected. You cannot edit this field.
See Chapter 5, Setting the Plate Layout or Auto Scale
Parameters, for details on the Auto Scale screen options.
Data format
Displays the current scaling choice.
Plate
Representation
At the bottom of the main setup screen, you see a plate map
with rows labeled “A” through “H” and columns labeled one to
12 for a 96-well plate. (The labels change as the numbering of
the wells change.) This area of the screen represents the
position of the wells in your plate. As CytoFluor scans the
plate, you can see the data collected for each well appear in
real time in the appropriate position on the screen. The top-left
of the screen displays the post processing selections in effect
for the run.
CAUTION
It is safer to give the file a name before you start a scan. If
the file has a name, the system saves each cycle to the file
as it completes the cycle. If you did not name the file, you
need to save it through the Save As… command under File
at the completion of the run. Otherwise, you could lose the
data if something disturbed the run (for example, a power
failure).
To set up multiple scans, see Section 3.3, Setting Up Multiple
Scans in a Cycle. Or, see Section 3.4, Saving Scan
Parameters, to save your settings. To start a scan, skip to
Section 3.6, Scanning Plates.
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