Analytik Jena Biometra TAdvanced 384, Operating Manual

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Biometra TAdvanced Protocol Wizard
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10.2 Enter a new polymerase or edit a polymerase
To enter program templates for new polymerases or to edit preset program templates
for polymerases press the corresponding button:
New polymerase
Edit polymerase
In the next screen (see Fig. 64) touch the corresponding input boxes to enter a name for
the polymerase and set parameters for all requested program steps. With two excep-
tions for most program steps the temperature and the hold time must be defined:

The temperature for the annealing step depends on the calculated primer melting
temperature (T
m
value) and has to be set individually for each primer pair (see sec-
tion "Primer Annealing Temperature Calculator" p. 87).

The hold time for the elongation step becomes calculated by the expected product
length.
The corresponding input boxes for the annealing temperature and elongation time are
shown in grey and cannot be activated.
Fig. 64 Edit polymerase screen
After creating the program template for a new polymerase or editing the program tem-
plate of a polymerase press the done button.
Done
In the next screen (see Fig. 65) touch the corresponding button to select a storage place
for the program template (polymerase). If the number of polymerases exceeds the