Agilent Technologies agilent 7683B, Installation, Operation And Maintenance Manual

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Troubleshooting
10
Installation, Operation, and Maintenance 173
Part 3, Maintenance and Troubleshooting
Symptom: Sample Carryover
Figure 72 Blank run showing carryover peaks
Table 29 Carryover problems
Possible cause Action
Number or type of washes is
insufficient. Check the run parameters for the number of sample and
solvent washes. The number of washes needed depends on
your application. See
“Sample Carryover” on page 79.
You ran out of solvent. Check the solvent bottles. If the solvent level is below 2.5 mL,
the syringe cannot reach the solvent. Replace the remaining
solvent with 4 to 4.5 mL of fresh solvent. See
“Preparing
Solvent and Waste Bottles” on page 116.
Check the waste bottles. If the waste level is near the neck of
the bottle, replace it with an empty bottle.
Syringe is worn or dirty. If the syringe look dirty or the plunger is sticking, clean the
syringe with an appropriate solvent or follow the syringe
manufacturer’s cleaning instructions. If the syringe seems
worn, replace it.
Samples (vial-to-vial) are of
immiscible types. In this situation, the sample and solvent washes may not
rinse the syringe properly. Increase the number of wash
cycles or use a solvent that rinses a variety of sample types.