AB Sciex QTRAP 4500 LC/MS/MS system, System User'S Manual, Page: 82 / 138

Operating Instructions — Analyze and Process Data
4500 Series of Instruments System User Guide
82 of 138 RUO-IDV-05-0264-A
Show TICs from a Spectrum
• Click Explore > Show > Show TIC .
The TIC opens in a new pane.
Show a Spectrum from a TIC
1. In a pane containing a TIC, select a range.
2. Click
Explore > Show > Show Spectrum .
The spectrum opens in a new pane.
Generate XICs
XICs can be generated only from single period, single experiment chromatograms or spectra. To
obtain an XIC from multi-period or multi-experiment data, split the data into separate panes by
clicking the triangle under the x-axis. For more information about using the available icons, refer
to Table 10-8 .
Several methods are available for extracting ions to generate an XIC, depending on whether
chromatographic or spectral data is used. Table 10-5 contains a summary of methods that can be
used with chromatograms and spectra.
Prerequisite Procedure
• Open Data Files on page 77 . Open a data file containing spectra.
Tip! To see an example data file, make sure that the Example project is selected.
Open the LIT folder, and then open the Reserpine.wiff file.
Tip! Right-click inside a pane containing a spectrum and then click Show
TIC .
Tip! Double-click in the TIC pane at a particular time to show the
spectrum.
Table 10-5 Summary of XIC Generation Methods
Method Use with
Chromatogram
Use with
Spectrum
Extraction
Selected range No Yes Extracts ions from a selected area in a
spectrum.
Maximum No Yes Extracts ions from a selected area in a
spectrum using the most intense peak
in the selected area. This option
creates an XIC using the maximum
mass from the selected spectral range.