AB Sciex QTRAP 4500 LC/MS/MS system, System User'S Manual, Page: 137 / 138

B
System User Guide 4500 Series of Instruments
RUO-IDV-05-0264-A 137 of 138
Calibration Ions and Solutions
a
Table B-1 Tuning Frequency
Calibration Resolution Optimization
Scan Type Frequency Manual/
Automated
Frequency Manual/Automated
Q1 and Q3 3 to 6 months Both 3 to 6 months Both
LIT Every 2 weeks;
as required
Both 3 to 6 months Automated only
Table B-2 Suggested Tuning Solutions for AB SCIEX Triple Quad™ 4500 System
System Positive Negative
AB SCIEX Triple Quad 4500 system 2 × 10
–6
M PPG (1:50) 3 × 10
–4
NEG PPG
Table B-3 Suggested Tuning Solutions for QTRAP
®
4500 LC/MS/MS System
Q1 and Q3 LIT
Instrument Positive Negative Positive and
Negative
QTRAP 4500 LC/MS/MS
system
2 × 10
–6
M PPG (1:50) 3 × 10
–4
NEG PPG 1:100 Agilent mix
Table B-4 Q1 and Q3 PPG Positive Ion Scans
Instrument Masses
AB SCIEX Triple Quad 4500
system
59.0 175.1 500.3 616.5 906.7 1254.9 1545.1 1952.4
QTRAP 4500 LC/MS/MS
system
59.0 175.1 500.3 616.5 906.7 1254.9 1545.1 1952.4
Table B-5 Q1 and Q3 PPG Negative Ion Scans
Instrument Masses
AB SCIEX Triple Quad
4500 system
45.0 411.2 585.4 933.6 1223.8 1572.1 1863.3 1979.3
QTRAP 4500 LC/MS/MS
system
45.0 411.2 585.4 933.6 1223.8 1572.1 1863.3 1979.3
Table B-6 Masses and Polarity for the QTRAP 4500 LC/MS/MS System (Agilent)
Instrument/
Polarity
Masses
LIT Positive 118.087 322.049 622.030 922.010 1521.972 – –
LIT Negative 112.985 431.982 601.978 1033.988 1633.949 – –