AB Sciex QTRAP 4500 LC/MS/MS system, System User'S Manual, Page: 53 / 138

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System User Guide 4500 Series of Instruments
RUO-IDV-05-0264-A 53 of 138
Operating Instructions — Acquisition
Methods
An acquisition method consists of experiments and periods. Use the Acquisition Method editor
to create a sequence of periods and experiments for the instrument and devices.
Create Acquisition Methods
Only devices configured in the active hardware profile appear in the Acquisition method pane.
Any devices added to the hardware profile must also be added to existing acquisition methods.
For more information about devices, refer to the Peripheral Devices Setup Guide.
1. In the navigation bar, under Acquire , double-click Build Acquisition Method .
2. In the Acquisition Method Properties tab, select a Synchronization Mode .
3. (Optional) Select the Auto-Equilibration check box and then type the desired
equilibration time, in minutes.
4. In the Acquisition method pane, click the Mass Spec icon.
5. On the MS tab, select a scan type.
6. Type values in the fields as required. Refer to Parameters on page 56 .
7. On the Advanced MS tab, type values in the fields as required. Refer to Parameters
on page 56 .
8. Click a device icon and then select the parameters for the device. To configure the
syringe pump, refer to Configure the Syringe Pump on page 53 .
9. Add any additional periods and experiments. Refer to Add an Experiment on
page 53 and Add a Period on page 54
10. Click File > Save .
Configure the Syringe Pump
1. In the Acquisition method pane, click the Syringe Pump icon.
The Syringe Pump Method Properties tab opens in the Acquisition Method
editor pane.
2. In the Syringe Diameter (mm) field, type the syringe diameter.
3. In the Flow Rate field, type the flow rate.
4. In the Unit list, select the units of flow.
Add an Experiment
1. Right-click the period and then click Add experiment .
An experiment is added below the last experiment in the period.
2. In the Acquisition Method Editor pane, select the appropriate device or instrument
parameters.