AB Sciex QTRAP 4500 LC/MS/MS system, System User'S Manual, Page: 50 / 138

Operating Instructions — Tune and Calibrate
4500 Series of Instruments System User Guide
50 of 138 RUO-IDV-05-0264-A
8. Select a Tuning Solution .
Depending on the solution selected, different modes are available.
i. Click a polarity.
ii. If available, click Q1 and Q3 in the Quad section. If available, click the
required scan speeds.
iii. If available, click the scan speeds in the LIT section.
iv. If available, click Excitation in the MS/MS/MS section.
9. Click Next .
10. If the Select a mode page opens, select Automatic .
11. Click Next .
12. Click GO .
The Verifying or Adjusting Performance screen opens. After the process has
completed, the Results Summary opens. For more information, refer to the Help.
13. If applicable (depending on the options selected), change solutions when prompted.
Verify or Adjust Performance
The top left corner shows the part of the instrument that is being tuned.
Current Spectrum : This graph shows the spectrum of the current scan, the optimal scan
selected by the software, or the scan at the current parameter value when the software results
are viewed in interactive mode.
The
Instrument Optimization Decision Plots , in the top right graph, dynamically show the
intensity versus voltage curves of the parameters that are currently being optimized.
Results Summary
The Results Summary is a record of any instrument settings changes that were made by the
Instrument Optimization software. Refer to the Results Summary shown in Figure 7-1 on
page 51 . This includes the location of data files and instrument settings backups, as well as step-
by-step changes and results during optimization.The Results Summary also shows a
verification report. This report contains a snapshot of the mass spectrum for each relevant mass
for the scan modes being verified. The spectrum is labelled with the target mass, where the mass
was found, mass shift, peak width, and peak intensity. The spectrum can be used as a visual
record of peak shape or scan mode performance. A summary table of results follows the spectra.
The
Results Summary is saved as a document in the folder indicated at the top of the report.
Users can print the Results Summary or open a previously saved Results Summary .