ThermoFisher Scientific Vanquish VC-D11, Руководство по эксплуатации, Страница: 133 / 192

7 • Maintenance and Service
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual Page 133
6. Push the isopropanol in the syringe into the flow cell. Make sure that
no residual air in the syringe is pushed into the flow cell.
7. Disconnect the waste line from the flow cell outlet.
8. Disconnect the flushing adapter from the flow cell inlet.
9. Install the flow cell plugs to the flow cell inlet and flow cell outlet.
10. Unscrew the flushing adapter from the syringe.
11. Make sure that the adapter and the syringe are empty and dry.
12. Put the protection cap onto the Viper adapter.
13. Pack and store the components of the kit in their original packaging.
14. If the flow cell is to be stored in its packaging
Remove the flow cell from the device and store it (see Removing the
Flow Cell ( } page 134) ).
7.7.2.1
Performing a Manual Injection
A sample can also be injected manually using the flushing and injection
kit.
Parts required
Flushing and injection kit for flow cells, including a syringe and a Viper
adapter
NOTICE
Particulate matter from the eluate can deposit in the flow cell and clog
it.
• Be careful not to inject any particulate matter into the flow cell.
• Only experienced users should perform a manual injection.
The manual injection requires setting suitable detection parameters,
starting acquisition, performing an autozero and data interpretation
afterward. The related injection procedures of autozero injection,
sample injection and flushing are similar to the flushing procedure
described in
Preparing the Flow Cell for Storage ( } page 132) .
Observe the following guidelines when performing a manual injection:
• Avoid injecting any particulate matter into the flow cell.
• All solvents used must be miscible.
• Flush the sample completely out of the flow cell after measurement.