ThermoFisher Scientific Vanquish VC-D11, Руководство по эксплуатации, Страница: 101 / 192

6 • Operation
Diode Array/Multiple-Wavelength Detectors (VC-D11, VC-D12, VF-D11)
Operating Manual Page 101
6.7.2.1 Selecting the Data Collection Rate
The data collection rate is the number of data points per second (Hz)
that the Chromeleon software collects from the detector and stores as
raw data.
When you select a data collection rate, observe the following guidelines:
• In general, each peak should be defined by at least 20 data points.
For chromatograms with co-eluting peaks or low signal-to-noise
ratios, 40 data points per peak are recommended.
• If the data collection rate is too low, the start points, maxima, and
end points of peaks will not be determined accurately.
• If the data collection rate is too high, data files may need more disk
space and post-run analyses may require more processing time.
• If all peaks are relatively wide, select a lower data collection rate (for
example, 1.0 Hz). This saves disk space and allows for a faster display
of data in the Chromeleon software.
• If any peaks of interest are less than a few seconds, select a higher
data collection rate (10.0 Hz, for example).
• Always consider the data collection rate and response time settings.
Set the two parameters together in order to optimize the amount of
data points collected, and reduce short-term noise, while still
maintaining peak height, symmetry, and resolution.