Siemens Rapidlab 1200, Руководство оператора, Страница: 428 / 448

E-68 Rapidlab 1200 Operator’s Guide: System Specifications
02087462 Rev.V
Method Comparison with Human Whole Blood Samples
For testing syringe and capillary modes, blood was collected in heparinized vacuum tubes.
It was tonometered at 37°C and/or adjusted chemically. Several levels of tHb were
prepared by separating the red cells from the plasma and recombining appropriately.
Specimens were assayed on Rapidlab 1265 and Rapidlab 865 systems.
Method comparison was quantified by obtaining estimates of proportional (slope), and
constant (intercept) bias, root mean square error (RMSE), and method correlation (R
square for r
2
coefficient of determination) by performing an ordinary least squares
analysis of the Rapidlab 1265 (dependent variable) versus the Rapidlab 865 (independent
variable). Results are summarized in the table below.
F HHb Syringe 62 4.53 -0.52 0.22
* tHb mode performance is equivalent to sample device used
† WRSD: within-run standard deviation
Table E-66 Rapidlab 1265 Human Whole Blood Method Comparison—pH
Mode N Slope Intercept RMSE r
2
Min Max
Capillary 117 0.996 0.036 0.006 0.999 7.052 7.691
Syringe 259 1.005 -0.030 0.009 0.998 7.010 7.696
Micro-
capillary 71 0.986 0.104 0.006 0.999 7.020 7.643
Micro-
syringe 145 0.991 0.064 0.006 0.999 7.022 7.646
pH Only
Capillary 122 1.003 -0.020 0.008 0.998 7.017 7.643
pH Only
Syringe 243 1.006 -0.051 0.008 0.998 7.012 7.645
pH, Glu,
Lac
Capillary 138 0.986 0.106 0.009 0.998 7.044 7.652
pH, Glu,
Lac
Syringe 274 0.991 0.070 0.008 0.998 7.054 7.661
Table E-67 Rapidlab 1265 Human Whole Blood Method Comparison— p CO
2
Mode N Slope Intercept RMSE r
2
Min Max
Capillary 198 1.014 0.211 3.179 0.998 7.0 238.2
Syringe 410 0.958 2.020 4.038 0.996 6.1 233.9
Table E-65 Rapidlab 1265 CO-ox Module Recovery and Precision with Total
Hemoglobin of ~20 g/dL and Oxyhemoglobin > 75%
Analyte Mode
*
n
Mean
Reference
Mean Bias WRSD
†