Test Procedures
To determine the percentage of Ammonia-Nitrogen that is unionized and ionized, consult
the Appendix.
PROCEDURE–SALT WATER
Use universal sample holder.
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
from menu.
3. Scroll to and select
ALL TESTS
(or another sequence containing
4 Ammonia-N L S
) from
TESTING MENU
.
4. Scroll to and select
4 Ammonia-N L S
from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with sample.
6. Insert tube into chamber, close lid and select
SCAN BLANK
. (See Note.)
7.
Remove tube from Spectro. Use the 1.0 mL plastic pipet (0354) to add 2.0 mL of
*Salicylate Ammonia #1 (3978). Cap and mix.
8. Use the 0.15 g spoon (0727) to add two measures of *Salicylate #2 Reagent (7457).
Cap and mix until dissolved. Wait 1 minute.
9. At end of 1 minute waiting period use 0.1 g spoon (0699) to add two measures
of *Salicylate #3 Reagent Powder (7458). Cap and shake vigorously for at least
30 seconds and all solid has dissolved. Wait 20 minutes for maximum color
development.
10. At the end of 20 minute waiting period, mix, insert tube into chamber, close lid and
select
SCAN SAMPLE
. Record result.
11. Press and hold
OFF
button to turn spectrophotometer off or press
EXIT
button to exit
to a previous menu or make another menu selection.
AMMONIA-NITROGEN,
Low Range
SMART Spectro 2 Test Procedures 05.02.16
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