Test Procedures
PROCEDURE
Use universal sample holder
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
.
3. Select
ALL TESTS
(or another sequence containing
47 H Peroxide-HR
) from
TESTING
MENU
.
4. Scroll to and select
47 H Peroxide-HR
from menu.
5. Use the pipet (0342) to add 5 drops of the sample water to a tube (0290).
6. Dilute to the 10 mL line with distilled or hydrogen peroxide-free water.
7.
Insert the tube into chamber, close lid and select
SCAN BLANK
.
8. Remove the tube from spectrophotometer and add 4 drops of *Hydrogen Peroxide
Reagent #1 (6452). Cap and mix.
9. Add one *Hydrogen Peroxide LR Tablet (6454A). Crush tablet with Tablet Crusher
(0175). Cap and mix for 30 seconds. Solution will turn pink if hydrogen peroxide is
present. Wait 5 minutes for full color development.
10. At the end of 5 minute waiting period, mix, insert tube into chamber, close lid and
select
SCAN SAMPLE
. Record result.
11. Press and hold
OFF
button to turn the spectrophotometer off or press
EXIT
button to
exit to a previous menu or make another menu selection.
NOTES: For best possible results, a blank should be determined to account for any
contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
For the most accurate results, the sample and reagents should be at 25 ±4°C.
SMART Spectro 2 Test Procedures 05.02.16
HYDROGEN PEROXIDE
, HIGH RANGE
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