Test Procedures
PROCEDURE
Use universal sample holder.
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
.
3. Scroll to and select
ALL TESTS
(or another sequence containing
53 Iron Phen
) from
TESTING MENU
.
4. Scroll to and select
53 Iron Phen
from menu.
5. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with sample.
6. Insert tube into chamber, close lid and select
SCAN BLANK
.
7.
Remove the tube from Spectro. Remove the cap and add 6 drops of *Acid
Phenanthroline Indicator (2776). Cap and invert the tube 4 times to mix reagents.
Wait fi ve minutes for maximum color development.
8. After
fi ve minutes, mix, insert tube into chamber, close lid and select
SCAN SAMPLE
.
Record result as ppm Ferrous Iron.
9. Remove tube from Spectro. Use the 0.1g spoon (0699) to add one measure of *Iron
Reducting Reagent (2777). Cap and invert 15-20 times to mix, wait 5 minutes for
maximum color delelopment.
10. After 5 minutes, mix insert tube into Spectro. Close lid and select
SCAN SAMPLE
.
Record result as ppm Total Iron.
11. Press and hold
OFF
button to turn spectrophotometer off or press
EXIT
button to exit
to a previous menu or make another menu selection.
12. Total Iron (ppm) - Ferrous Iron (ppm) = Ferric Iron (ppm)
NOTE: For best possible results, a reagent blank should be determined to account for any
contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
SMART Spectro 2 Test Procedures 05.02.16
IRON
, I,I0-PHENANTHROLINE
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