Test Procedures
AMMONIA-NITROGEN, HIGH RANGE
NESSLERIZATION METHOD • CODE 3642-SC
QUANTITY
CONTENTS
CODE
30 mL
Ammonia Nitrogen Reagent #1
V-4797-G
2 x 30 mL
*Ammonia Nitrogen Reagent #2
*V-4798-G
1
Pipet, 1 mL, plastic
0354
*WARNING:
Reagents marked with an * are considered to be potential health hazards. To
view or print a Safety Data Sheet (SDS) for these reagents go to www.lamotte.com.
Search for the four digit reagent code number listed on the reagent label, in the contents
list or in the test procedures. Omit any letter that follows or precedes the four digit code
number. For example, if the code is 4450WT-H, search 4450. To obtain a printed copy,
contact LaMotte by email, phone or fax.
Emergency information for all LaMotte reagents is available from Chem-Tel:
(US, 1-800-255-3924) (International, call collect, 813-248-0585).
Ammonia nitrogen is present in various concentrations in many surface and ground water
supplies. Any sudden change in the concentration of ammonia nitrogen in a water supply is
cause for suspicion. A product of microbiological activity, ammonia nitrogen is sometimes
accepted as chemical evidence of pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups that
produce nitrite and nitrate. This oxidation requires that dissolved oxygen be available in the
water. Ammonia is an additional source of nitrogen as a nutrient which may contribute to
the expanded growth of undesirable algae and other forms of plant growth that overload
the natural system and cause pollution.
APPLICATION:
Drinking, surface, and saline waters; domestic and industrial
wastes.
RANGE:
0.00–4.00 ppm Ammonia Nitrogen
MDL:
0.05 ppm
METHOD:
Ammonia forms a colored complex with Nessler’s Reagent in
proportion to the amount of ammonia present in the sample.
Rochelle salt is added to prevent precipitation of calcium or
magnesium in undistilled samples.
SAMPLE HANDLING &
PRESERVATION:
Ammonia solutions tend to be unstable and should be
analyzed immediately. Sample may be stored for 24 hours at
4°C or 28 days at –20°C.
INTERFERENCES:
Sample turbidity and color may interfere. Turbidity may be
removed by a fi ltration procedure. Color interference may be
eliminated by blanking the instrument with a sample blank.
SMART Spectro 2 Test Procedures 05.02.16
AMMONIA-NITROGEN,
High Range
www.
GlobalScientificSupply
.com
GLOBAL SCIENTIFIC SUPPLY
The Laboratory Supply Company!
