Test Procedures
NOTE: HR Reagent must be made fresh each week. If reagent is refrigerated, it may be kept
up to 3 weeks.
PROCEDURE–HIGH RANGE
Use universal sample holder.
A. PREPARATION OF HR REAGENT
NOTE: The quantity of Indigo Blue Stock Solution (3989) supplied will prepare one batch of
HR Reagent for the High Range Ozone procedure or fi ve batches of HR Reagent for the Low
Range Ozone procedure.
1. Use the 50 mL graduated cylinder to carefully add 35 mL of Ozone Buff er (3991) to
amber glass bottle marked HR Reagent (3988-MT-G).
2. Use the 50 mL graduated cylinder to carefully add 15 mL of Indigo Blue Stock Solution
(3989) to the amber glass bottle. Cap and mix.
B. DETERMINATION OF OZONE
3. Use the 1.0 mL transfer pipet (2-2170) and pump (30527) to add 1.0 mL of HR
Reagent (3988) to each of 2 clean tubes (0290).
4. If chlorine is present add 3 drops Chlorine Inhibitor (3990) to each tube. Cap
tubes.
5. Take one of the prepared tubes (0290) and sampling apparatus (0681) to sampling
site.
6. Lower end of tubing of sampling apparatus to desired depth. Slowly withdraw and
depress plunger several times to purge syringe and tubing. Slowly withdraw plunger to
fi ll purged syringe.
7.
Remove plastic tubing from syringe. Remove cap from the prepared tube. Place tip of
syringe against inside of the prepared tube. Slowly depress plunger and fi ll to the 10
mL line and cap. This is the Sample Tube.
NOTE: DO NOT SHAKE OR INVERT THE SAMPLE.
8. Fill the second prepared tube (0290) to the 10 mL line with ozone free water. This is
the Reagent Blank.
9. Press
ON
button to turn spectrophotometer on.
10. Scroll to and select
PROGRAMMED TESTS
.
11. Scroll to and select
ALL TESTS
(or another sequence containing
72 Ozone HR
) from
TESTING MENU
.
12. Scroll to and select
72 Ozone HR
from menu.
13. Insert the Reagent Blank tube into chamber, close lid and select
SCAN BLANK.
SMART Spectro 2 Test Procedures 07.05.16
OZONE
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