Test Procedures
PROCEDURE
Use universal sample holder.
NOTE: Place Dispenser Cap (0692) on *Mixed Acid Reagent (V-6278). Save this cap for
refi ll reagents.
1. Press
ON
button to turn spectrophotometer on.
2. Scroll to and select
PROGRAMMED TESTS
.
3. Scroll to and select
ALL TESTS
(or another sequence containing
64 Nitrate-N LR
) from
TESTING MENU
.
4. Scroll to and select
64 Nitrate-N LR
from menu.
5. Rinse a clean tube (0290) with sample water. Fill to 10 mL line with sample.
6. Insert tube into chamber, close lid and select
SCAN BLANK
.
7.
Remove tube from Spectro and pour off 5 mL into graduated cylinder or similar.
Discard the remaining sample.
8. Pour the 5 mL sample from a graduated cylinder or similar into the tube. Use the
graduated cylinder or similar to measure 5 mL of *Mixed Acid Reagent (V-6278) and
add to tube. Cap and mix. Wait 2 minutes before proceeding to Step 9.
9. Use the 0.1 g spoon (0699) to add two measures of *Nitrate Reducing Reagent (V-
6279). Cap.
10. Hold tube by index fi nger and thumb and mix by inverting approximately 60 times a
minute for four minutes. Wait 10 minutes for maximum color development.
NOTE: At end of waiting period an undissolved portion of Nitrate Reducing Reagent
may remain in bottom of the tube without aff ecting results.
11. At the end of the 10 minute waiting period, mix, insert tube into chamber, close lid and
select
SCAN SAMPLE
. Record result.
12. Press and hold
OFF
button to turn spectrophotometer off or press
EXIT
button to exit
to a previous menu or make another menu selection.
NOTES: For best possible results, a reagent blank should be determined to account for
any contribution to the test result by the reagent system. To determine the reagent blank,
follow the above test procedure to scan a distilled or deionized water blank. Then follow
the above procedure to perform the test on a distilled or deionized water sample. This test
result is the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only when a new lot
number of reagents is obtained.
To convert Nitrate Nitrogen (NO
3
–N) results to ppm Nitrate (NO
3
), multiply by 4.4.
SMART Spectro 2 Test Procedures 05.02.16
NITRATE-NITROGEN
, LOW RANGE
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