u
For 48 samples, add 4.5 ml RA1
u
For 96 samples, add 9 ml RA1
4
At the robot PC, select Run.
5
Remove the MSA1 plate from the robot deck.
6
Apply a foil seal to the MSA1 plate using the heat sealer.
7
Incubate in the preheated Illumina Hybridization Oven for 1 hour.
8
Vortex the plate at 1800 rpm for 1 minute.
9
Check to make sure that the pellets are resuspended. If necessary, repeat the incubation and vortexing
steps.
10 Pulse centrifuge at 280 × g.
SAFE STOPPING POINT
If you are stopping, store sealed MSA1 plate(s) at 2°C to 8°C for up to 24 hours. If more than 24 hours, store
at -25°C to -15°C.
Store sealed RA1 at -25°C to -15°C. If RA1 will be used the next day, seal it, and store it overnight at 4°C.
Hybridize to BeadChip
This step dispenses the fragmented, resuspended DNA onto BeadChips. Incubation then hybridizes each
DNA sample to a section of the BeadChip.
Consumables
u
1% aqueous Alconox solution
u
100% EtOH
u
DI H
2
O
u
PB2 (3 tubes (1 tube per 4 BeadChips))
u
XC4
Preparation
1
If frozen, thaw the MSA1 plate at room temperature, and then centrifuge at 280 × g for 1 minute.
2
Remove BeadChips from storage, but do not unpackage.
3
Preheat the heat block to 95°C.
4
Preheat the Illumina Hybridization Oven to 48°C.
Prepare Robot Tip Alignment Guide
1
Make sure you have the correct robot tip alignment guide for the assay.
The barcode says Guide-E
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
46
Infinium HD Super Assay Reference Guide