12 Remove and discard the cap mat.
13 Place the MSA1 plate back on the robot bed according to the bed map.
14 When prompted, select OK.
15 Remove the MSA1 plate from the robot bed and carefully seal with a
new, dry
cap mat. Avoid shaking
the plate until the cap mat is seated.
16 Invert 10 times to mix.
17 Incubate at 4°C for 30 minutes.
18 Place in the centrifuge opposite another plate of equal weight.
19 Centrifuge at 3000 × g for 20 minutes.
u
When centrifuging is complete, proceed
immediately
to the next step to avoid dislodging the blue
pellet.
u
If a delay occurs, repeat the 20 minute centrifuge.
20 Remove MSA1 plate from centrifuge.
21 Make sure that a blue pellet is present in the bottom of each sample well.
22 Remove and discard the cap mat.
23 Hold the plate over an absorbent pad and do as follows.
a
Quickly invert to decant the supernatant.
b
Drain liquid onto the absorbent pad, and then smack the plate down. Avoid the liquid drained onto
the pad.
24 Keeping the plate inverted, firmly tap until all wells are free of liquid (~1 minute). Do not allow supernatant
to pour into other wells.
25 Place the uncovered, inverted plate on a tube rack for 1 hour at room temperature to air-dry the pellet.
26 Make sure that a blue pellet is still present in the bottom of each sample well.
SAFE STOPPING POINT
If you are stopping, seal the plate(s), and store at -25°C to -15°C.
Resuspend DNA
This step uses RA1 to resuspend the precipitated DNA.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
44
Infinium HD Super Assay Reference Guide