7
Remove and discard the cap mat.
8
Add 300 μl 100% 2-propanol to each sample well.
9
Carefully seal with a
new, dry
cap mat. Avoid shaking the plate until the cap mat is seated.
10 Invert the plate 10 times to mix.
11 Incubate in a refrigerator set at 4°C for 30 minutes.
12 Centrifuge at 3000 × g at 4°C for 20 minutes.
13 Immediately remove the plate from the centrifuge.
u
When centrifuging is complete, proceed
immediately
to avoid dislodging the blue pellets.
u
If a delay occurs, repeat the 20 minute centrifuge.
14 Make sure that a blue pellet is present in the bottom of each sample well.
15 Remove and discard the cap mat.
16 Hold the plate over an absorbent pad and do as follows.
a
Quickly invert to decant the supernatant.
b
Drain liquid onto the absorbent pad, and then smack the plate down on a dry area of the pad.
17 Keeping the plate inverted, firmly tap until all wells are free of liquid (~1 minute). Do not allow supernatant
to pour in to other wells.
18 Place the uncovered, inverted plate on the tube rack for 1 hour at room temperature to air-dry the
pellets.
19 Make sure that a blue pellet is still present in the bottom of each sample well.
20 Keeping the plate inverted, use a Kimwipe to remove any residual alcohol draining from the wells of the
plate or remaining on the surface of the plate.
CAUTION
Do not overdry the pellets. Pellets that are overdried are difficult to resuspend and can lead to poor data
quality.
SAFE STOPPING POINT
If you are stopping, seal the plate, and store at -25°C to -15°C for up to 24 hours.
Document # 11322427 v03
For Research Use Only. Not for use in diagnostic procedures.
12
Infinium HD Super Assay Reference Guide