Gambro COBE 2991, Справочник по основам

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6-24 COBE 2991 Cell Processor • Essentials Guide
Processing and Common Operations
5
If predilution is not required, proceed to step 6. Or, to predilute the starting
cell product, perform the following steps:
a Clamp the clear tubing between the RCD and the rotating seal
(Figure 6-16 on page 6-18).
b Place the cell product bag on the tabletop (Figure 6-17 on page 6-19).
c Open the clamp on the blue-striped tubing.
d Press the BLOOD IN button. Valve V1 opens, allowing processing/wash
solution to flow by gravity into the cell product bag. Manually agitate the
cell product bag to ensure adequate mixing (Figure 6-18 on page 6-20).
e When the desired volume of processing/wash solution has entered the cell
product bag, press the STOP RESET button to close valve V1.
f Close the slide clamp on the blue-striped tubing.
g Allow the mixture to equilibrate (if necessary) for the appropriate time.
h Remove the clamp from the tubing between the rotating seal and the
RCD.
6
Hang the cell product bag on the left hanger bar.
7
Open the slide clamp on the blue-striped tubing and let the starting cell
product flow by gravity into the cell processing bag.
After a short time, the starting cell product stops flowing. Depending on the
initial volume, some starting cell product may be left in the cell product bag.
Note: DO NOT
press the AIR OUT
button for this
procedure.
8
Set the CENTRIFUGE SPEED control to 3000 rpm, then press the START
SPIN button. The centrifuge spins, pushing air and starting cell product back
up the blue-striped tubing to the cell product bag.
9
Press the STOP RESET button when air and starting cell product reach the
cell product bag. Additional starting cell product fills the cell processing bag.