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LB 983 NightOWL II Operating Manual

System Description

77

In the application definition you can select the excitation and emission filter and the lamp energy
for fluorescence measurements. For best results we recommend a lamp energy between 80
and 100%. Available filters can be selected depending on which filter wheel / slider configura-
tion you have selected in the <

Excitation

> and <

Emission

>section.

The Berthold Application Support is available to recommend filters when you are selecting the
optimum filter combination for your fluorescence application.

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Содержание NightOWL II LB 983

Страница 1: ...BA2 Rev No 05 These units are not designed for use in hazardous areas The units must not be repaired by any person other than Berthold Technologies service engineers or technicians authorized by Berth...

Страница 2: ...Operating Manual LB 983 NightOWL II Berthold Technologies GmbH Co KG Calmbacher Str 22 75323 Bad Wildbad Germany Phone 49 7081 177 0 Fax 49 7081 177 100 bio Berthold com www Berthold com bio...

Страница 3: ...e Acquisition Device 71 5 3 Acquisition Chamber 73 6 INDIGO SOFTWARE DOCUMENTATION 83 6 1 Graphical User Interface 84 6 2 Application Toolbar 84 6 3 Identity Context 85 6 4 Identities and Measurements...

Страница 4: ...LB 983 NightOWL II Operating Manual Contents 10 7 Index 132...

Страница 5: ...in bold type inside angular brackets Start Stop Enumerations are emphasized by Action steps are identified by 1 2 Warning signs at the instrument We Wear eye protection When using the transilluminato...

Страница 6: ......

Страница 7: ...everything possible to guarantee that the equipment functions safely both electrically and mechanically The user has to make sure that the instrument will be set up and installed properly to guarantee...

Страница 8: ...ed in this user guide There are no exchangeable electrical components in the instrument In case of malfunction call authorized service personnel Use only parts described in this manual for servicing D...

Страница 9: ...ke sure that the operator remains safe and that the instrument con tinues to work correctly Any service and maintenance work not described in this user guide has to be performed by authorized service...

Страница 10: ...nel comp tent Tous les utilisateurs des appareils sont tenus de lire d abord ces instruc tions de service Ne mettez jamais des parties de votre corps ou des objets dans l appareil lorsque celui ci est...

Страница 11: ...sont plus activ es cap t et parties de la fa ade de l appareil Faites attention aux parties mobiles L int rieur de l appareil et certaines pi ce peuvent at teindre des temp ratures pouvant provoquer d...

Страница 12: ...respondant dans ce mode d emploi Le fonctionnement correcte ne peut tre garantit qu la condition que des pi ces de rechange appropri es sont utilis es Degr de pollution est de niveau 2 Ne pas utiliser...

Страница 13: ...nutzer muss daf r sorgen dass die Ger te so aufgestellt und installiert werden dass ihr si cherer Gebrauch nicht beeintr chtigt wird Die Ger te sind werkgepr ft und wurden in betriebssicherem Zustand...

Страница 14: ...ufuhr zu unterbrechen Um das Ger t vollkommen vom Netz zu trennen kann das Netzkabel gezogen werden Ger t ausschalten bevor der Stecker gezogen wird Wenn das Ger t ge ffnet ist sind Sicherheitsma nahm...

Страница 15: ...Injektorfl ssigkeit darf nur dann vorgenommen werden wenn die entsprechenden Reagenzbeh lter angeschlossen sind Beachten Sie alle gesetzlichen Vorschriften f r den Umgang mit biolo gischem Abfall mit...

Страница 16: ......

Страница 17: ...author ized representative or user d parts other than original Berthold Technologies parts are used e the product and parts thereof have been altered without written authorization from Berthold Techno...

Страница 18: ...GmbH Co KG Technical Support Calmbacher Str 22 75323 Bad Wildbad Germany Phone 49 7081 177 114 Fax 49 7081 177 301 Email service berthold com At the end of this manual you will find a Customer Reply...

Страница 19: ...rst Time Initialization Setup 24 4 1 11First Time Initialization Setup 26 4 1 12User Management 30 4 1 13Installing Fluorescence Filters 31 4 1 14Filter Management 32 4 1 15Filter Inventory 33 4 1 16F...

Страница 20: ...width of workplace the load carrying capacity of the table or the desktop should be approx 100 kg The table or desktop should stand firmly and not wobble Network connection for use of network resourc...

Страница 21: ...camera has been installed After power on the device is initialized and the camera is moved up to the home position Thus installation of the camera without using the IndiGO software is not possible If...

Страница 22: ...e cameras mentioned above the NC100em does not communicate via a stand ard interface This model uses a special interface card which is plugged into the PCI bus of the PC The card is installed into a f...

Страница 23: ...e rear panel via a RJ 45 network cable with the network interface card of the PC It does not matter if you are using a regular patch cable or a crossed patch cable cross over cable since the cable typ...

Страница 24: ...tOWL and PC is to be preferred since interferences may occur in the network due to data traffic You need an Ethernet card RJ 45 in the PC to connect the NightOWL II Most PCs already have such a port o...

Страница 25: ...tion The interface card requires an additional power supply in the PC which is provided via a 4 pole standard AMP connector which is also used in DVD drives If only S ATA power supply con nectors are...

Страница 26: ...ram Click on the file SETUP EXE on the data carrier After launch ing the installation program a welcome screen appears After the welcome screen the folder for the software installation must be defined...

Страница 27: ...up will install device drivers for the instrument hard ware on your computer the Windows system might show a warning that a signature of a Windows driver cannot be verified In this case allow the setu...

Страница 28: ...ices If the calibration for your device was stored on the Instrument by Berthold Technologies before it was delivered you can download the stored calibration parameters from the instruments flash memo...

Страница 29: ...tion users filters and calibration files If there is a backup available chose the command that suits you best Select to continue To maintain the instrument the instruments controller needs to create a...

Страница 30: ...first time After a reboot it will not start automatically With instruments running unattended for a longer period it is recommended to choose the se cond option Start controller with Windows to have...

Страница 31: ...he existing database You can browse for a suitable database lo cation through the browse command Please note IndiGO manages all measuring data in a file based database The indiGO software is able to m...

Страница 32: ...p the correspondending IP address or the host name like 192 168 1 2 1024 or IMAGING_PC 1024 The IP port number to contact the instrument controller is al ways 1024 Host and port number must be separat...

Страница 33: ...account tick the box Set a default user account By selecting Next you will be asked to set up a user name and a passwort Please note that the created account will have administrator rights Click to c...

Страница 34: ...to all measurements created Administrators can change the ownership of measurements and man age invitations of all users Service Service users can change all settings of the system New users can be c...

Страница 35: ...with the device An arrow is printed on the fluorescence filter housing With excitation filters this arrow should face the sample i e away from the source of light For emission filters filter wheel th...

Страница 36: ...simply grab the center knob and pull out the wheel carefully The wheel is fixed by a magnet To reinstall simply slide the filter until the wheel snaps into the magnetic holder A initialization is requ...

Страница 37: ...for the emission filters The inventory lists can be reached via Excitation Inventory Filter Inventory for the exci tations filters or via Emission Inventory Filter Inventory for the emission filters T...

Страница 38: ...it the charging of a slider or a wheel select Edit The charging can be changed using the filter droplist Filter N Please keep in mind that only available and unused filters are displayed in the dropli...

Страница 39: ...ement 1 Measurement n Identity n Measurement 1 Measurement n Project n Projects and Users Projects can be associated to users When created the project belongs to a specified owner The owner has the po...

Страница 40: ...p an application in indiGO Administor access rights are necessary If this is not guaran teed the function to define an application is not available to rule out faulty operations To set up a new applic...

Страница 41: ...ge while high gain gives higher intensity numbers but limited dynamic range Read out defines the speed to read out the camera High speed is recommended for phgotographioc images high intensity measure...

Страница 42: ...the lowest intensities on the image Flatfield Correction corrects the uneven image caused by the vignetting of the lens Just enable the cosmic suppression to avoid cosmic spots during longer exposures...

Страница 43: ...100ms exposure time 10 illumination intensity can be used for most applications Disable all Post Processors in the corresponding section The close iris feature will improve image quality depth of focu...

Страница 44: ...the sample height is 0 mm If elevated samples are meas ured e g animals or plants the sample height has to be set to a relevant range e g height of a special leaf If you are analyzing animals it is ad...

Страница 45: ...is used which is 1 to 1024 in X and Y The MultiSample feature allows the possibility to split the image into sections Each section is treated as a single sample identity This feature might be useful...

Страница 46: ...refer to Applications Templates Measuring Tem plates Select New Controller Template from the Controller Templates context menu indiGO starts the wizard and you first have to enter a name for the contr...

Страница 47: ...ightOWL systems This command sets the LED illumination panel to a defined intensitiy The used intensi ties are temporarily saved for future use Daylight Off Not available on NightOWL systems Turns off...

Страница 48: ...e power socket inside the dark room Relay 1 Relay 2 is not used yet Wait Defines a waiting time within the control sequence No other action is possible while waiting Sample Changer Not available on Ni...

Страница 49: ...parameters like user access rights scale settings etc To create a new project indiGO starts the wizard The following parameters are necessary Project Name Enter the project name under which the projec...

Страница 50: ...ect the Acquisition Settings used for the project You can define a Pre Measurement and a Post Measurement template to be executed be fore and after the measurement Under Measurement you select the app...

Страница 51: ...d users are listed in the left column and can be added to the project using the add command All users assigned to the project are listed in the right column Users can be removed using the remove comma...

Страница 52: ...details about how to use the scheduler can be found at the end of the chapter To start an acquisition refer to Acquisition Scheduling Start an Acquisition The acquisition wizard will be shown The pro...

Страница 53: ...tart the actual measurement sequence select Upon completion of the measure ment the data is automatically stored and displayed on the screen The measurement consists of a photographic image in graysca...

Страница 54: ...quence refer to Acquisition Scheduling Create a new Schedule The sequence wizard will be shown First of all the type of scheduled event must be specified For Scheduled measurements choose Schedule Mea...

Страница 55: ...e scheduler Now the sequence is active and the instrument controller is taking care of the measurements It s possible to evaluate other measurements or even exit indigo Only the instrument controller...

Страница 56: ...menu Please note The contents of the project folder shown depend on the user account based on the indiGO user access management The identity number is displayed on top of the data view If several sam...

Страница 57: ...h the associated sliders With these sliders the scaling of the pseudocolor signal image can be set The top slider defines which intensity value has been assigned to the highest color value Values abov...

Страница 58: ...At the bottom margin of the screen a transparency slider is available This slider can be used to define the transparency of the overlay If the slider is in the 100 position the overlay is com pletely...

Страница 59: ...ings as default for new Measurements is selected these settings are also valid for all future measurements in the project The selections Annotation Layer and Manually Layer will copy the areas and ann...

Страница 60: ...e measured data This function is recommendet to evaluate the measuring data To use this function select An additional window is opened where the line profile created from a given lead is displayed The...

Страница 61: ...hic image only please refer to associated command The same applies if you want to create a 3D surface plot from the measurement data only From the dialog Photo Properties or Data Properties select Ran...

Страница 62: ...of all automatically found peaks in the current image To run a new search select The following parameters can be edited o Peak Threshold Based on Using a droplist different search algorhytms are avail...

Страница 63: ...eaks To start the peaksearch select 4 3 6 Define Areas Manually The Manual Areas are available via the command Manually They are used for the manual definition of integration areas Here you have the p...

Страница 64: ...shape to the required position A context menu for all shapes is available To open the context menu select the shape and right click inside the shape For editing the standard clipboard commands are ava...

Страница 65: ...LB 983 NightOWL II Operating Manual Quick Start 61 To export areas the Export command from the Overlay Properties can be used To import areas the Import command from the Overlay Properties can be used...

Страница 66: ...owing options can be set Create a Front Page This will create a report front page The contents of the page like your company name address or logo can be setup through the indiGO options Export Images...

Страница 67: ...LB 983 NightOWL II Operating Manual Quick Start 63...

Страница 68: ...he Report menu will start the Excel Report Creation Wizard To create a report follow the instructions given by the wizard In brief the following options can be set Create a Front Page This will create...

Страница 69: ...contain one to three worksheets depending on the selected options IndiGO The IndiGO sheet contains the front page Analysis The Analysis sheet contains all measurement results and the diagrams dependin...

Страница 70: ...cheduling Create a new Schedule The system will start the Create a new Schedule wizard which allows you to select the con tents you want to schedule first To schedule a measurement select Schedule Mea...

Страница 71: ...t 15 15 00 The Overall Measurements are set to 2 and the Delay Cycle Time was set to 2 as well Therefore the system will add two measurements into the scheduler The first will be executed Wednesday th...

Страница 72: ...LB 983 NightOWL II Operating Manual Quick Start 68 Note If you open the selected project and the scheduled identity the measured image data will be displayed automatically too...

Страница 73: ...h Door Button 73 5 3 2 Illumination of the Acquisition Chamber Dark Box 73 5 3 3 Connector Field 73 5 3 4 Camera and Lens 74 5 3 5 Camera Transport Unit 74 5 3 6 Camera Lift 75 5 3 7 Filter Wheel 75 5...

Страница 74: ...orking with UV lamps or transilluminators NightOWL II LB 983 comprises the following com ponents o Acquisition device including the transport mechanism with camera and lens and the electronics The hig...

Страница 75: ...ight source for fluorescence measurements are accommodated in the upper part of the device The ventilation cap is designed for drawing off the waste heat caused by the camera cooling Do not move the c...

Страница 76: ...rt unit camera lens and filter wheel o Connector field o Outlet of the fluorescence light for connection of the illumination light guide Rear panel Top part o Connection ports o On off switch o Mains...

Страница 77: ...of the Acquisition Chamber Dark Box Four LED s installed on the walls of the dark box ensure constant illumination of the acquisition chamber However the illumination is dependent on the camera setti...

Страница 78: ...hould keep this setting since any modification will result in a deterioration of the light flow rate Discharge electrostatics before taking out the camera Clean the camera and lens only with special c...

Страница 79: ...e bottom plate to have access to the spindle which moves the camera lift Do not reach into the instrument while the camera lift is moving Risk of injury 5 3 7 Filter Wheel The filter wheel is installe...

Страница 80: ...to the device and to initialize it After the excitation filter has been loaded you have to calibrate the filter in order to use the light source optimally The filter calibration is part of the service...

Страница 81: ...measurements For best results we recommend a lamp energy between 80 and 100 Available filters can be selected depending on which filter wheel slider configura tion you have selected in the Excitation...

Страница 82: ...anesthesia which are available as extras and the line light for example increase the sample height by 40 mm If the sample is placed on such a table you have to enter 40 mm as minimum height If you ar...

Страница 83: ...tions on the Rear Panel see chapter 8 2 1 for information on how to replace the fuse and how to clean the air filter CE I 0 1 2 3 CE I 0 Ports and air filter Discharge electrostatics before touching C...

Страница 84: ...e camera several centimeters above the bottom plate Discharge electrostatics before carrying out any work Open the door of the acquisition chamber to check the movement of the camera transport unit Ch...

Страница 85: ...ector o Unscrew the camera from the C mount adapter o Move the camera z axis into the low position step 98000 using the service soft ware or indiGO Click on select Position and choose Z Axis and Posit...

Страница 86: ......

Страница 87: ...ndiGO Menu 96 6 8 1 New 96 6 8 2 Start a new Acquisition 96 6 8 3 Create a new Schedule 96 6 8 4 Report 96 6 8 5 Print Image 96 6 8 6 Print Report 97 6 8 7 Logoff 97 6 8 8 Exit 97 6 9 Tools Menu 98 6...

Страница 88: ...ollowing commands Emergency Stop Stops all axis of the connected NightOWL The operation will cancel the currently running Measurement Might force a reset to the NightOWL Start an Acquisition Start a n...

Страница 89: ...line help About Display program version and copyright information 6 3 Identity Context On the left of the context bar the actual selected identity name is displayed On the right are three quick comman...

Страница 90: ...image and contrast settings as well as the overlay proper ties of the annotation and the manual layers can be applied to all measurements of the cur rent identity You also have the possibility to star...

Страница 91: ...r With the contrast slider on the left side of the image the brightness of the photographic display is defined The top handle sets the upper brightness value whereas the bottom handle is for the defin...

Страница 92: ...ort he scaling and the alignment Scale n Scale the contrast of the photo using the selected algorithm Align Align the contrast sliders using the selected algorithm 6 5 2 Photo Properties Dialog Photo...

Страница 93: ...ate the photo was taken In our example 03 March 2010 005800 is the recording time In our example 12 58 00 AM By following the link the indiGO image viewer will be opened and the photo will be displaye...

Страница 94: ...been assigned to the highest valued color Values above this threshold are displayed in white The bottom slider defines which intensity in the signal image is displayed with the lowest valued color All...

Страница 95: ...LB 983 NightOWL II Operating Manual indiGO Software Documentation 91 For further analysis a LUT and a surface is supported In addition the overly intensity can be set through Overlay level...

Страница 96: ...the over lay The following options are available False Color Table Activates the system default false color table Record Filter Activates the filter properties used for recording Custom Filer Enables...

Страница 97: ...om Increase the zoom Used to increment the zoom Current zoom Used to setup the zoom manually displays current zoom factor Align Align image into the view used to calculate a best fit zoom factor Undo...

Страница 98: ...LB 983 NightOWL II Operating Manual indiGO Software Documentation 94 Manually Menu Create manual areas Peak Search Menu Starts the peak search Peak Search Command...

Страница 99: ...ort Rich Text Report Starts the wizard to export a Rich Text report Same function as Rich Text Report Copy current view to clipboard The current presentation of the measurement will be copied to the c...

Страница 100: ...easurements Several quick check commands are available to verify the settings made to the application For further details please refer to chapter 4 2 5 6 8 3 Create a new Schedule The system will star...

Страница 101: ...port indiGO offers the possibility to create and display measurement reports The output can be adapted to the user s requirements For further details please refer to chapter 4 4 6 8 7 Logoff This comm...

Страница 102: ...twork drive or removable storage to assure a proper backup An Backup name can be assigned This is the name of the file created by the backup extension zip dba Depending on the amount of data and the c...

Страница 103: ...ith WinLight can be imported into indiGO for reanaly sis via this command A smart wizard will guide you through the steps necessary to import the data Please follow the instructions given by the wizar...

Страница 104: ...n 100 6 10Service Menu 6 10 1 Filter Manager This command will start the indiGO filter manager For further details please refer to section 4 1 13 6 10 2 User Manager This command will start the indiGO...

Страница 105: ...r NightOwl device and to perform all system calibration tasks 6 10 4 Device State The Device State command can be used to display the current states of the instrument the camera and the scheduler Raw...

Страница 106: ...Position can be used to move the Z drive to a position where the camera can be accessed After clicking the buttons a warning message comes up Clicking on OK will move the camera drive to the dedicate...

Страница 107: ...form the quickcheck 6 10 7 Controller Version The command will display the current controller and instrument version information Please note Watch the owl while moving the mouse 6 10 8 Messages The Me...

Страница 108: ...tion type can be changed This might be required when the com puter was moved away from the system or in case the database was changed from the local disk to a network drive Enter the type of connectio...

Страница 109: ...ing indiGO The user management can be disabled completely or a default user who logs on automatically at program start can be assigned First Time Initialisation Wizard This button will start the initi...

Страница 110: ...This option is not persistent and the toolbar will be shown on the next start of indiGO 6 11 2 Statusbar This command shows or hides the status bar This option is not persistent and the statusbar wil...

Страница 111: ...xit 97 6 9 Tools Menu 98 6 9 1 Backup Projects 98 6 9 2 External Tools 98 6 9 3 Import indiGO 1 x Project 98 6 9 4 Import Winlight Data 99 6 10 Service Menu 100 6 10 1Filter Manager 100 6 10 2User Man...

Страница 112: ...ally by switching off the instrument and move the camera drive manually This approach should be chosen if the drive unit got stuck in this case click on Proceed as follows to remedy a collision 7 1 2...

Страница 113: ...ed table etc from the baseplate of the NightOWL Switch on the NightOWl and wait until the init procedure is finished Due to the blocked motor the init will be finished after a 2 minute timeout Launch...

Страница 114: ...D3D device error pops up instead of the surface plot This indicates that the installed graphics card or the corresponding driver does not support Microsoft Windows DirectX 9 features In this case a dr...

Страница 115: ...g and Maintenance 8 1 Camera and Lens 112 8 1 1 Cleaning the Camera Box Removing the Bottom Plate 112 8 1 2 Cleaning the Camera 112 8 1 3 Cleaning the Lens 113 8 2 Darkbox 114 8 2 1 Replacing the Air...

Страница 116: ...ate o Open the door of the acquisition chamber to check the movement of the camera transport unit Check that there is no sample tray or any other device which may block the camera movement o Do not re...

Страница 117: ...o the specified position o Pull off the filter wheel from below so that the un derside of the camera lens is accessible o Clean the underside of the objective Make sure there are no residues dust clea...

Страница 118: ...he fuse inset with the screwdriver like a drawer o Replace the fuse depending on the line voltage o Insert the fuse holder again until it clicks into place Use only fuses that match the value and the...

Страница 119: ...ation Lens NC 100 standard 25mm f 0 95 NC 320 standard 25mm f 1 6 C Mount adapter Sample chamber Illumination internal illumination can be adjusted via software white light fluorescence illumination 4...

Страница 120: ......

Страница 121: ...e power supply specified on the type label of the device Never leave the transilluminator powered up without any supervision Pull the power cord before carrying out maintenance and repair work and mak...

Страница 122: ...sity of the light source These safety glasses en sure that bright intense light intensity will not be harmful to the eye of the beholder All safety glasses that reduce the light intensity by approx 50...

Страница 123: ...er on of the NightOWL the heating system is detected and may get initialized If the heating system is plugged in during ongoing operation if will work only after the device has booted up again After a...

Страница 124: ...section of the dark box of the NightOWL It ensures reliable light coupling In addition the connection can be secured against inadvertently coming loose by the toggle screw at the transfer port 10 1 5...

Страница 125: ...te place it on the NightOWL base plate and center the plate in such a way that the 2 fitting bolts fit in the 2 holes at the plate Connect the 2 gas suppy tubes with the inner part of the gas flange t...

Страница 126: ...icroplate position E9 emits light with an energy of approx 20nW This spot can be used to check the NightOWL calibration The measuring time should not exceed 1 second since the camera could be saturate...

Страница 127: ...r a central supply An input pressure of approx 1 2 bar is required The max flow rate is about 3 liters per minute at full ca pacity of the system The tubing consists of a special oxygen tube with an i...

Страница 128: ...steel valves at the vaporizer rack Both outlets NightOWL and induction box lead to the carbon filters of the vacuum controller The vacuum controller is driven by the pressured air as well A vacuum is...

Страница 129: ...n the inox bean holder is a 3 way gas distributor Turn on the floodgate that stream the induction box o Adjust AIR flow meter to 1 liter minute maximum 3 liters minute o Adjust vaporizer to 4 maximum...

Страница 130: ...n off oxygen flow meter o Move animal s from NightOWL to waking box o Turn off mask s exhauster o Check all floodgates of stainless steel beam holder and NightOWL are closed o Turn off compressor wait...

Страница 131: ...the home position Remove the air adapters from the camera open the 2 thumb screws left and right of the camera unplug the USB cable and Power supply and carefully take out the camera Fix the transport...

Страница 132: ...LB 983 NightOWL II Operating Manual Appendix 128 Noise Killer hood Foam block...

Страница 133: ...Send Customer Reply Form to Berthold Technologies GmbH Co KG Technical Support Calmbacher Str 22 75323 Bad Wildbad Germany Phone 49 7081 177 114 Fax 49 7081 177 301 Email service berthold com or your...

Страница 134: ...s Address Phone Fax Email Instrument ID no Serial no Embedded software version Instrument driver software version Accessory instruments PC Software PC software version Windows version Computer type CP...

Страница 135: ...an envelope attached to the out side please use capital letters instrument component serial no instrument or component has come into contact with radioactive substances Isotope s means of decontaminat...

Страница 136: ...ase Connection 104 Decontamination 131 demo installation 24 Export Report Settings 105 Exposure Time 37 Filter Manager 34 filter slider 76 filter wheel 75 first time initialization 27 Flatfield Correc...

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