Thermo Scientific Vanquish VH-P10-A-02 Operating Manual Download Page 210

Page: 210 / 238

•

Troubleshooting

Page 210

Vanquish Pumps (VH-P10-A-02) Operating Manual

8.5

Pressure Problems or Shifts in Retention Time

8.5.1

Introduction

When you observe pressure issues or shifts in retention time, the
following sections help you to identify and eliminate the source for the
problem:

•

Pressure pulsation
See

section 8.5.2, page 211

and

section 8.5.4, page 214.

•

Shift in retention times
See

section 8.5.2, page 211

•

Messages regarding the pump head pressure or nonlinear pressure
sensor appear in chromatography software
See

section 8.5.3, page 213.

•

Inconstant pressure or high backpressure at the column and pump
See

section 8.5.4, page 214.

Summary of Contents for Vanquish VH-P10-A-02

Page 1: ...Vanquish Pumps VH P10 A 02 Operating Manual 4820 4411 EN Revision 1 0 October 2017 thermoscientific ...

Page 2: ...ization of Thermo Fisher Scientific Inc This manual is provided as is The contents of this manual are subject to being changed without notice in future revisions Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free Thermo Fisher Scientific Inc assumes no responsibility and will not be liable for any errors omissions damage or loss that might r...

Page 3: ...or sales support for HPLC products contact your local Thermo Fisher Scientific sales organization For contact information go to Contact Us on http www thermofisher com Technical Assistance For technical support for HPLC products contact your local Thermo Fisher Scientific support organization For contact information go to Contact Us on http www thermofisher com ...

Page 4: ...Contacting Us Page 4 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 5: ...rving this Manual 19 2 1 3 Safety Symbols on the Pump 19 2 1 4 Rating Plate 20 2 2 Intended Use 21 2 3 Safety Precautions 22 2 3 1 General Safety Information 22 2 3 2 Qualification of the Personnel 23 2 3 3 Personal Protective Equipment 23 2 3 4 Electrical Safety Precautions 24 2 3 5 General Residual Hazards 25 2 3 6 In Case of Emergency 26 2 4 Solvent and Additive Information 27 2 5 Compliance In...

Page 6: ...ing Capillaries and Tubing Through the System 60 5 6 3 Connecting Fittings Capillaries and Tubing 62 5 6 4 Guiding Liquids to Waste 64 5 6 5 Connecting the Solvent Lines 65 5 6 6 Setting Up the Seal Wash System 69 5 6 7 Connecting the Pump and Autosampler 77 5 7 Turning On the Pump 78 5 8 Setting Up the Pump in the Software 79 6 Operation 81 6 1 Introduction to this Chapter 82 6 2 Safety Guideline...

Page 7: ...7 4 2 Flushing the Pump 122 7 4 3 Cleaning or Decontaminating the Pump 124 7 4 4 Predictive Performance 126 7 5 Seal Wash System 128 7 5 1 Testing the Seal Wash System for Leakage 128 7 5 2 Replacing the Seal Wash Lines 129 7 5 3 Replacing the Seal Wash Detector 132 7 6 Y Connector 134 7 7 Pump Head 136 7 7 1 Overview of Pump Head Parts 137 7 7 2 Replacing the Pump Head 138 7 7 3 Replacing the Pis...

Page 8: ... 7 15 Replacing the Doors 184 7 16 Transporting or Shipping the Pump 186 7 16 1 Preparing the Pump for Transport 187 7 16 2 Transporting the Pump to a New Location 188 7 16 3 Shipping the Pump 188 7 17 Replacing the Slide In Module 190 7 17 1 Removing the Slide In Module 190 7 17 2 Installing the Slide In Module 192 8 Troubleshooting 195 8 1 General Information about Troubleshooting 196 8 2 Messag...

Page 9: ...eneral Information 220 10 2 Ship Kit 221 10 3 Optional Accessories 222 10 4 Consumables and Replacement Parts 223 11 Appendix 227 11 1 Compliance Information 228 11 1 1 Declarations of Conformity 228 11 1 2 WEEE Compliance 229 11 1 3 FCC Compliance 229 11 1 4 Manual Release History 229 11 2 Digital I O 230 12 Index 233 ...

Page 10: ...Contents Page 10 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 11: ...mps VH P10 A 02 Operating Manual Page 11 1 Using this Manual This chapter provides information about this manual the conventions used throughout the manual and the reference documentation that is available in addition to this manual ...

Page 12: ...event personal injury damage to the pump or loss of data when followed properly Note the following The pump configuration may vary therefore not all descriptions necessarily apply to your particular pump If some detail applies to only one model or variant the model or variant is identified by name Illustrations in this manual are provided for basic understanding They can vary from the actual model...

Page 13: ...section or procedure appear in the section or procedure to which they apply They appear different from the main flow of text Safety messages are often preceded by an alert symbol and or alert word The alert word appears in uppercase letters and in bold type Make sure that you understand and follow all safety messages presented in this manual 1 2 2 Special Notices and Informational Notes Special no...

Page 14: ...ermo Chromeleon 7 Services Manager Start Instrument Controller References and Messages References to additional documentation appear italicized Messages that appear on the screen are identified by quotation marks Viewpoint If not otherwise stated the expressions left and right in this manual always refer to the viewpoint of a person that is facing the pump from the front Particularly Important Wor...

Page 15: ...eader or Adobe Acrobat is required Software Documentation Additional software documentation includes the following Chromeleon 7 Help and documents The Chromeleon 7 Help provides extensive information and comprehensive reference material for all aspects of the software For basic information about device installation and configuration refer to the Installation Guide for specific information about a ...

Page 16: ...erating Manual TIP The Chromeleon Help and documents are included in the software shipment Third Party Documentation Refer also to the user documentation provided by the manufacturers of third party components and materials for example Safety Data Sheets SDSs ...

Page 17: ...2 Safety Vanquish Pumps VH P10 A 02 Operating Manual Page 17 2 Safety This chapter provides general and specific safety information and informs about the intended use of the pump ...

Page 18: ...he pump The safety symbols and signal words in this manual include the following Always be aware of the safety information Do not proceed until you have fully understood the information and consider the consequences of what you are doing CAUTION Indicates a hazardous situation that if not avoided could result in minor or moderate injury WARNING Indicates a hazardous situation that if not avoided c...

Page 19: ...s manual and pass it on to any subsequent user Read understand and comply with all safety messages and precautionary statements presented in this manual 2 1 3 Safety Symbols on the Pump The table lists the safety symbols that appear on the pump or on labels affixed to the pump Follow the safety notices in this manual to prevent the risk of operator injury or damage to the pump Symbol Description I...

Page 20: ...e serial number part number module name revision number if any line and fuse rating and the manufacturer s address TIP An additional type label on the leak tray of the pump indicates the module name serial number part number and revision number if any To facilitate device identification have the information from this label available when communicating with Thermo Fisher Scientific ...

Page 21: ...stem are intended for laboratory research use only They are not intended for use in diagnostic procedures Laboratory Practice Thermo Fisher Scientific recommends that the laboratory in which the Vanquish system is used follow best practices for LC analyses This includes among others Using appropriate standards Regularly running calibration Establishing shelf life limits and following them for all ...

Page 22: ...ents options and peripherals specifically authorized and qualified for the pump by Thermo Fisher Scientific Perform only the procedures that are described in this operating manual and in supporting documents for the pump Follow all instructions step by step and use the tools recommended for the procedure Open the enclosure of the pump and other components only if specifically instructed to do so i...

Page 23: ...ule the installer is responsible for ensuring the safety of the module and system General Operation The pump is designed to be operated only by trained and qualified personnel in a laboratory environment All users must know the hazards presented by the pump and the substances they are using All users should observe the related Safety Data Sheets SDSs 2 3 3 Personal Protective Equipment Wear person...

Page 24: ... 4 Electrical Safety Precautions WARNING Electric Shock or Damage to the Device High voltages are present inside the device that could cause an electric shock or damage to the device Do not make any changes to the electrical or grounding connections If you suspect any kind of electrical damage disconnect the power cord and contact Thermo Fisher Scientific Technical Support for assistance Do not op...

Page 25: ...volume of substances to the minimum volume required for sample analysis Do not operate the pump in a potentially flammable environment Avoid accumulation of harmful substances Make sure that the installation site is well ventilated Dispose of hazardous waste in an environmentally safe manner that is consistent with local regulations Follow a regulated approved waste disposal program WARNING Biohaz...

Page 26: ...erate the pump in the presence of flammable gases or fumes CAUTION Allergic Reaction Some capillaries in the Vanquish system are made of MP35N a nickel cobalt based alloy Individuals with sensitivity to nickel cobalt may show an allergic reaction from skin contact CAUTION Sparking due to Electrostatic Discharge Liquid flowing through capillaries can generate static electricity This effect is parti...

Page 27: ...ines and recommendations on the use of solvents and additives in the chromatography system see section 6 5 page 89 Allowed ranges and concentrations standard system configuration pH range 2 12 Chloride concentration 1 mol L or less Observe the following pH values higher than 9 5 with optical detectors Avoid using mobile phases with a pH value higher than 9 5 together with optical detectors This ca...

Page 28: ... the specific solvent and additive information for this detector Refer to the operating manual for the Vanquish charged aerosol detector Follow any specific recommendations presented in other sections of this manual Refer also to the operating manuals for all modules in the Vanquish system They may provide additional guidelines and information ...

Page 29: ...ls see section 11 1 page 228 Changes that you make to the device may void compliance with one or more of these EMC and safety standards Changes to the device include replacing a part or adding components options or peripherals not specifically authorized and qualified for the product by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and addi...

Page 30: ...2 Safety Page 30 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 31: ...3 Pump Overview Vanquish Pumps VH P10 A 02 Operating Manual Page 31 3 Pump Overview This chapter introduces you to the pump and the main components ...

Page 32: ... of pistons and piston seals The piston seal wash system keeps the pistons wet and rinses the piston seals automatically This prevents crystallization and particle deposits on the piston surfaces and seals which may damage the components Solvent selector 2 from 6 for increased flexibility Purge unit for easy and automatic purging Built in vacuum degasser for enhanced flow stability and best detect...

Page 33: ... electronic compressibility compensation The picture illustrates how the pump operates Figure 1 Operating principle No Description 1 Solvent selector pump inlet 2 Solvent degasser vacuum degasser 3 Pump head left two pistons parallel 4 Y connector left 5 Pump head right two pistons parallel 6 Y connector right 7 Purge unit 8 System pressure sensor 9 Inline filter static filter 10 Pump outlet outle...

Page 34: ...essible liquids The flow rate at atmospheric pressure is always kept constant Two 3 port solvent selectors allow forming binary gradients from three solvents per channel The solvent to be used for each channel is selected in the user interface The vacuum degasser enhances the flow stability and allows best detector sensitivity by continuously removing air that may be trapped in the solvents The so...

Page 35: ...g clips for routing solvent lines to the pump heads 4 Pump head right 5 Leak sensor 6 Type label indicating the module name serial number part number and revision number if any 7 Drain port 8 Seal wash detector drop detector 9 Vacuum degasser solvent B partly hidden by seal wash detector 10 Solvent selector solvent B 11 Vacuum degasser solvent A 12 Solvent selector solvent A 13 Seal wash pump 14 T...

Page 36: ...3 Pump Overview Page 36 Vanquish Pumps VH P10 A 02 Operating Manual No Description 17 Purge unit 18 Y connector left 19 System pressure sensor ...

Page 37: ...e pistons and seals by preventing crystallization and particle deposits on the piston surfaces and seals which may otherwise damage the pistons and seals The picture shows the flow path through the seal wash system Figure 3 Flow path through the seal wash system No Description 1 Supply line for seal wash liquid from autosampler 2 Seal wash pump peristaltic pump 3 Peristaltic tubing PharMed tubing ...

Page 38: ...tomatic purging The pump is shipped from the manufacturing site with the capillaries and tubing installed to the purge unit Figure 4 Connections on purge unit No Description 1 Capillary from left Y connector 2 Capillary to system pressure sensor long capillary 3 Capillary to inline filter 4 Capillary from right Y connector 5 Waste tubing from purge unit to drain port 6 Capillary from system pressu...

Page 39: ...uid is collected in the leak tray and guided to the drain port From the drain port the liquid is discharged to waste through the drain system of the Vanquish system When the leak sensor detects leakage the status indicators change to red and beeping starts to alert you Follow the instructions in this manual to find and eliminate the source for the leakage If leakage is not resolved after 3 minutes...

Page 40: ... and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the pump are available in the Chromeleon Help TIP The pump can be operated also with other data systems such as Xcalibur In this case installation of additional software is required in addition to the data system software For details contact the Thermo Fishe...

Page 41: ...4 Unpacking Vanquish Pumps VH P10 A 02 Operating Manual Page 41 4 Unpacking This chapter provides information for unpacking the pump and informs you about the scope of delivery ...

Page 42: ...te for the damage only if reported immediately Unpacking the Pump CAUTION Heavy Load Bulky Device The pump is too heavy or bulky for one person alone to handle safely To avoid personal injury or damage to the pump observe the following guidelines Physical handling of the pump including lifting or moving requires a team effort of two persons Use the carrying handles that were shipped with the pump ...

Page 43: ...aterial Leave any protective films attached to the surfaces of the pump until it is properly positioned in the system stack 6 Transport the pump by the carrying handles to the installation site if it is not already there and place it in the system stack see the System Arrangement section in this operating manual 7 On each carrying handle loosen the attachment screw until the carrying handle is mov...

Page 44: ...rying handles with the attachment screws and all packing material These items will be needed if the pump is transported to a new location or shipped 9 Some surfaces including the doors of the pump are covered by a protective film during shipment Remove the protective film from all surfaces as applicable ...

Page 45: ...umps VH P10 A 02 Operating Manual Page 45 4 2 Scope of Delivery The following items are included in the delivery Pump Ship Kit For details about the kit content see section 10 2 Ship Kit page 221 Operating manual Power cord ...

Page 46: ...4 Unpacking Page 46 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 47: ...H P10 A 02 Operating Manual Page 47 5 Installation This chapter specifies the requirements for the installation site and describes how to set up install and configure the pump in the Vanquish system and in the chromatography software ...

Page 48: ...ee page 22 CAUTION Heavy Load Bulky Device The pump is too heavy or bulky for one person alone to handle safely To avoid personal injury or damage to the pump observe the following guidelines Physical handling of the pump including lifting or moving requires a team effort of two persons Use the carrying handles that were shipped with the pump to move or transport the pump Never move or lift the pu...

Page 49: ...on to the safety guidelines and observe all site requirements For the safety guidelines when installing the pump see section 5 1 page 48 For the site requirements see section 5 3 page 51 2 Set up the pump hardware See section 5 5 page 54 3 Set up the flow connections See section 5 6 page 59 4 Turn on the pump See section 5 7 page 78 TIP Before turning on the power to a Vanquish system module for t...

Page 50: ... are using and or perform the qualification manually The Instruments Installation Qualification Operating Instructions provide information about the parameters to be adapted and the required settings 7 Recommended Perform Operational Qualification The qualification kit includes all materials required for the qualification and detailed instructions Moving the Pump after Installation If you have to ...

Page 51: ...e ranging capability accepting any line voltage in the range specified for the device CAUTION Electric Shock or Damage to the Device Connecting the device to a line voltage higher or lower than specified could result in personal injury or damage to the device Therefore connect the device to the specified line voltage only Never connect the device to a power socket that is shared with other equipme...

Page 52: ...t has a protective ground connection In case of emergency it must be possible to reach the power cord easily at any time to disconnect the device from the power line WARNING Electric Shock or Damage to a Product Misuse of the power cords could cause personal injury or damage the instrument Use the power cords provided by Thermo Fisher Scientific only for the purpose for which they are intended Do ...

Page 53: ... Accessing the Interior Components To access the interior components in the pump open the front doors To allow easy access from the front the user accessible components and flow connections in the pump are located directly behind the doors Figure 7 Opening the pump doors ...

Page 54: ...rovides information about the pump connectors and cables 5 5 1 System Arrangement The pump is part of the Vanquish system The system modules are typically arranged in a system stack with the arrangement depending on the system configuration Figure 8 Vanquish system standard configuration example No Description 1 Solvent Rack 2 Detector 3 Autosampler ...

Page 55: ...ation Vanquish Pumps VH P10 A 02 Operating Manual Page 55 No Description 4 Pump 5 System Base 6 Column Compartment For instructions on how to set up the system stack refer to the Vanquish System Operating Manual ...

Page 56: ...d device connectors on the pump No Description 1 Rating plate indicating the serial number part number module name revision number if any line and fuse rating and the manufacturer s address 2 3 4 Main power switch on off control Fuse holder Power inlet connector 5 System Interlink port Allows power on off control for the pump from the Vanquish system base and device communication ...

Page 57: ...g the USB ports only as described above If the USB ports are used for any other purpose Thermo Fisher Scientific cannot ensure proper functionality Connecting the Pump NOTICE Never use defective communication cables If you suspect that a cable is defective replace the cable To ensure trouble free operation use only the cables provided by Thermo Fisher Scientific for connecting the pump 1 Place the...

Page 58: ...sation is present in the device Condensation in the device can damage the electronics If you suspect that condensation is present allow the device to warm up to room temperature This may take several hours Wait until the condensation is completely gone before proceeding 5 Connect the free end of the power cord to an appropriate power source ...

Page 59: ...e chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the components only on a clean lint free surface Keep your tools clean Use only lint free cloth for cleaning For installation instructions and handling guidelines for capillaries tubing and fittings see se...

Page 60: ...in the Vanquish System Operating Manual 5 6 2 Guiding Capillaries and Tubing Through the System Flow connections between the modules of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices Tubing Chase Tubing Guide Tubing Bracket To guide certain tubes and lines solvent tubing wash liquid tubing detector waste line from ...

Page 61: ...needle wash 4 Detector waste line Tubing brackets are available for holding the tubing in place Slip the bracket side onto the drain pipe Figure 11 Tubing bracket left tubing bracket installed right Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules Route flow connections from one module to the next module in the Vanquish system...

Page 62: ...s Even minute particles may cause damage to the system or lead to invalid test results Do not install capillaries or tubes that are stressed nicked kinked or otherwise damaged Install capillaries and fittings only at the positions for which they are intended 5 6 3 2 Connecting Viper Capillaries This section describes how to connect Viper capillaries All Viper flow connections in the Vanquish syste...

Page 63: ... the slot in the knurl For narrow connections you can easily remove the knurls from neighboring capillaries through this slot and attach them again later 3 If the connection leaks a Tighten the connection a little more b If leakage continues remove the capillary c Clean the capillary ends carefully by using a lint free tissue wetted with isopropanol d Reinstall the capillary e If the connection co...

Page 64: ...ish system To avoid false leak alarms verify that the waste line sits properly in the waste outlet port If it does not liquid from the purge unit may accumulate in the drain port and activate the leak sensor Figure 13 Outlets for guiding liquids to waste view from top No Description 1 Waste line from purge unit routing path to outlet 2 Drain port with 2a Drain port wall 2b Groove in drain port wal...

Page 65: ...luding reservoir reservoir cap cap plugs and retaining guide for the solvent line Solvent lines Solvent line filters Optional to shut off the solvent flow through the system at the solvent reservoir Shut off valve kit containing one shut off valve and fitting connections Tubing cutter If you want to install shut off valves after initial setup or operation of the pump follow the steps in section 6 ...

Page 66: ...detector assembly funnel and detector Grasp and pull the assembly upward by the funnel The detector including the tubing and cable can remain connected to the funnel Figure 15 Removing the seal wash detector assembly 2 Route the solvent lines from the pump inlet to the solvent rack through the tubing guides in the pump and all modules above the pump in the system stack Observe the routing guidelin...

Page 67: ... Solvent lines up to three solvent lines 3 4 Not to be used for solvent lines reserved for other tubing 4 Prepare the solvent lines a Feed each solvent line through a retaining guide b Feed the solvent line through an open hole in a cap for a solvent reservoir The retaining guide keeps the solvent line in place in the reservoir c Close any open holes in the reservoir caps with the cap plugs Figure...

Page 68: ... for rinsing 8 Tighten the reservoir caps hand tight Press the retaining guide into the hole in the reservoir cap to ensure that the tubing is kept in place in the cap 9 Optional Required only if you want to install a shut off valve a Cut the solvent line near the reservoir cap with a tubing cutter Make sure that the cut is at a right angle to the length of the line b Onto the free end of each sol...

Page 69: ...istaltic pump seal wash lines and seal wash detector drop detector In the autosampler Seal wash lines Reservoir for the seal wash liquid Both the autosampler and pump are shipped with the seal wash components installed and the lines connected within each of the two modules To complete the setup interconnect the seal wash paths of the modules connect and fill the seal wash reservoir and flush the s...

Page 70: ...ash line silicone tubing for connection to the seal wash pump the line is pre installed to the seal wash outlet of the metering device head Seal wash line silicone tubing for connection to the seal wash reservoir TIP To avoid the formation of gas bubbles in the seal wash reservoir place the reservoir in the system stack on the same level or higher than the autosampler The setup description below a...

Page 71: ... No Description 1 Seal wash reservoir 2 Seal wash lines in the autosampler thick tubing 2a Wash line from reservoir to inlet port 2b Seal wash inlet port right tubing connector 2c Wash line from inlet port to metering device head 2d Wash line to seal wash pump 3 Metering device head 4 Seal wash pump peristaltic pump ...

Page 72: ... head to seal wash detector 6 Seal wash detector drop detector Follow these steps 1 In the pump engage the peristaltic tubing PharMed tubing in the seal wash pump Figure 21 Seal wash pump No Description 1 Pump lever 2 Pump rotor 3 Tubing holder peristaltic tubing 4 Tubing connectors 5 Seal wash line from autosampler 6 Peristaltic tubing 7 Seal wash tubing to pump head a Press down the lever on the...

Page 73: ...e holder 2 In the autosampler the seal wash line to the pump and the seal wash inlet port right tubing connector are interconnected Disconnect the seal wash line clear silicone tubing from the seal wash inlet port right tubing connector Figure 22 Seal wash connections in the autosampler 3 Route the seal wash line from the seal wash outlet of the metering device through the tubing guides to the per...

Page 74: ...em stack 8 Route the seal wash line through the guide hole in the solvent rack Figure 23 Guide hole and tubing guides in the solvent rack No Description A Guide hole 1 2 Not to be used for wash liquid line reserved for other tubing 3 Tubing guide for wash liquid line 4 Not to be used for wash liquid line reserved for other tubing 9 Prepare the seal wash line a Feed the seal wash line through the r...

Page 75: ...ir in the solvent rack Position the seal wash lines straight in the tubing guides 13 Check the seal wash lines across the entire flow path Make sure that the lines are not bent pinched or squeezed at any point in the flow path 14 Flush the seal wash system see the next section 5 6 6 3 Flushing the Seal Wash System To flush the seal wash system select one of the following alternatives Turn on the p...

Page 76: ...ect the tubing from the seal wash detector Figure 25 Seal wash detector 2 Insert a syringe into the open end of the tubing 3 To allow the liquid to travel easily through the seal wash system press down the lever of the seal wash pump and hold it in that position with one hand 4 Pull out the syringe plunger to draw seal wash liquid into the tubing 5 Release the lever of the seal wash pump when the ...

Page 77: ...n the autosampler and pump left view from autosampler right view from pump No Description 1 Capillary connecting the pump and autosampler 1a Capillary end connected to the autosampler injection valve 1b Capillary end to be connected to pump outlet 2 Guide holes in autosampler housing and pump housing 2a Guide hole in autosampler housing view from above 2b Guide hole in pump housing view from below...

Page 78: ...stem base 2 Turn on the pump with its main power switch Turn the pump off with the main power switch when instructed to do so for example during maintenance Pressing the system power button will not be sufficient to turn off the power to the device completely Whenever the power to the pump is turned on the following sequence of events occurs The pump performs a self test If the self test is not su...

Page 79: ...romatography software is already installed on the data system computer and a valid license is available For more information about setting up the Vanquish system in the software refer to the Vanquish System Operating Manual The Help for the software that you are using provides detailed information about the settings on each property page ...

Page 80: ...5 Installation Page 80 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 81: ...6 Operation Vanquish Pumps VH P10 A 02 Operating Manual Page 81 6 Operation This chapter describes the elements for pump control provides information for routine operation and for shutdown ...

Page 82: ...ump has already been completed If this is not the case refer to the instructions in chapter 5 Installation before proceeding see page 47 For a basic description of instrument control and automated sample analysis with the Chromeleon software refer to the Vanquish System Operating Manual Details on control and operation of the pump are available in the Chromeleon Help ...

Page 83: ... Pay attention also to the following guidelines When operating the chromatography system always set the lower pressure limit for the pump This prevents damage resulting from leakage or from running the pump dry If there is evidence of leakage in the pump turn off the pump flow and remedy the situation immediately If the pump flow is interrupted act appropriately to protect the components in the de...

Page 84: ...LED on the keypad provide a quick visual check of the operational status of the pump 6 3 1 Keypad The keypad inside the pump allows you to perform certain functions directly from the pump When you press a button a short beep confirms that the function is performed When the pump is connected in the Chromeleon software some functions may not be available from the keypad see further down in this sect...

Page 85: ...ect the pump head for which you want to perform the action DOCK Pressing the button moves the pistons of the selected pump head to the appropriate position for maintenance procedures such as pump head or piston replacement and undocks the pistons When undocking is complete the LED next to the FLOW button starts flashing green for the selected pump head The LED continues flashing while no pistons a...

Page 86: ...e beep for the current alarm In addition the Select function remains available from the keypad allowing you to select a pump head 6 3 2 Status Indicators The status LED bar on the front side of the pump and the STATUS LED on the inside keypad provide information about the pump status LED Bar The LED bar provides the information when the pump is closed When the pump is connected in the Chromeleon s...

Page 87: ...or remedial action see the Troubleshooting section in this operating manual STATUS LED The STATUS LED on the keypad inside the pump provides the following information STATUS LED Description Off dark The power to the pump is turned off Green The pump is functioning properly Red A problem or error has occurred For the related message check the Chromeleon Audit Trail For remedial action see the Troub...

Page 88: ...s off the system power button stands out If the main power switch on a device is off you cannot turn on the device with the system power button To turn off a device completely you have to turn it off with the main power switch on the device Pressing the system power button will not be sufficient to turn off the power to the device completely Whenever the power to the pump is turned on the followin...

Page 89: ...d accordingly by the vendor When preparing salt solutions or buffers and preparation is complete use membrane filtration 0 2 µm to remove any particulate matter and to reduce microbial growth When using water use high quality water for example UHPLC grade or LC MS grade 0 2 µm filtered When you use water from water purification systems keep in mind that polymeric contamination may occur if the pur...

Page 90: ... as solvents can cause swelling and damage the seals For best seal performance do not recycle or recirculate solvents Avoid using methanol from aluminum reservoirs To reduce the growth of algae consider using amber glassware or using appropriate additives for example formic acid After operation flush out buffers and solutions that form peroxides Never leave buffers salt solutions or aggressive sol...

Page 91: ...ish modules follow the instructions in the Vanquish System Operating Manual Some components of the pump are filled with isopropanol when the pump is shipped from the manufacturing site When operating the pump for the first time use solvents that are miscible with isopropanol If they are not use an appropriate intermediate solvent To remove the isopropanol from the pump components Verify that the f...

Page 92: ...partment and post column cooler Sample compartment thermostatting in the autosampler Flow cell in a fluorescence detector Evaporation tube in a charged aerosol detector Turning on the lamp or lamps in the UV VIS detector Monitoring the pump pressure and pressure ripple and checking that the pressure is stable and the ripple within reasonable limits for the application Monitoring the detector signa...

Page 93: ...pward Convex curves cause rapid changes in solvent composition at the beginning of the gradient and slower changes at the end Slope changes over time become extreme as curves go from 4 least convex to 1 most convex Curves 6 through 9 are concave upward Concave curves cause slower changes in solvent composition at the beginning of the gradient and rapid changes at the end Slope changes over time be...

Page 94: ...en the pump is shipped Leak Sensor Mode Enabled This is the preferred setting Piston calibration value The Piston Calibration Value property must match the 3 digit calibration value that is imprinted on the rear of the piston You have to update the value in the Chromeleon software for example when you install new pistons see page 144 Piston seal washing rear seal washing The seal wash system is en...

Page 95: ...its the Chromeleon software stops the pump flow and aborts a running queue or batch Pump pressure In the dialog box for the pump in the Chromeleon 7 Instrument Configuration Manager Chromeleon 6 8 Server Configuration the Pump_Pressure check box is selected as a standard when the pump is set up in Chromeleon The Chromeleon software uses this setting to generate the channel for recording the pump p...

Page 96: ... volume Examples To deliver 100 of solvent B2 select B2 and set B to 100 To deliver 100 of solvent A3 select A3 and set B to 0 To deliver 30 of solvent A1 and 70 of solvent B3 select A1 and B3 and set B to 70 Solvent consumption You can monitor the solvent consumption when you have entered the following information for the related solvent Volume of solvent in the reservoir at the beginning of a se...

Page 97: ...the waste container at the beginning of a sequence Upper limit for the liquid level in the waste container The Remain Time property for the waste reports the time left until the liquid level is expected to reach the upper limit The Chromeleon software calculates the time from the current flow rate and calculated current liquid level Warning limit when you want to be informed about the liquid level...

Page 98: ...e pulsation High noise level or pulsation during pump operation Non reproducible analysis When changing to a different type of solvent for example when changing to a different analytical method Items required Solvents suitable for purging To flush out the isopropanol when operating the pump for the first time use solvents that are miscible with isopropanol If they are not use an appropriate interm...

Page 99: ...solvent by setting A_Selector to A1 or A2 or A3 For channel B set B_Selector to B1 or B2 or B3 2 Determine the percentage of solvent that you want to deliver by setting the percentage for solvent B B Solvent A automatically delivers the remaining volume Recommended The channel to be purged should deliver 100 of the volume Examples To purge channel B with solvent B1 delivering 100 select B1 and set...

Page 100: ... selected each channel is purged with the most recently used solvent delivering 50 of the volume When the pump is operated for the first time the purge cycle will be performed for solvent 1 of the selected pump head A1 or B1 respectively Recommended The channel to be purged should deliver 100 of the volume Check and adapt the setting in Chromeleon if considered necessary 2 Optional in Chromeleon A...

Page 101: ... or solvent additives For TFA trifluoroacetic acid applications TIP Installing a mixing system affects the gradient delay volume dwell volume and ripple see section 6 9 2 page 102 Always use the built in vacuum degasser Purge the pump when changing to a different type of solvent or when restarting the pump after periods of inactivity Monitor the usage of specific pump components that are subject t...

Page 102: ... Thus the gradient delay volume of an HPLC system is the volume that the pump must deliver until a change in solvent composition reaches the column inlet Figure 28 Gradient delay volume of an HPLC system No Description 1 Pump with 1a Point where the gradient is formed purge unit 2 Autosampler 3 Column 4 Detector 5 Gradient delay volume of the system The pump contributes to the system gradient dela...

Page 103: ...the inline filter 11 Dwell volume of the pump In the standard configuration the pump has been optimized for the dwell volume and ripple You can adapt the dwell volume or ripple to your requirements by replacing the inline filter and capillary mixer with which the pump is shipped with a mixing system Operating the pump with a mixing system reduces the ripple but increases the dwell volume of the pu...

Page 104: ...ystem The table lists the available mixing systems Description Mixing system volume 200 µL comprising Static mixer volume 150 µL Capillary mixer volume 50 µL Mixing system volume 400 µL comprising Static mixer volume 350 µL Capillary mixer volume 50 µL For ordering information see section 10 3 Optional Accessories page 222 To install the mixing system follow the steps in section 6 9 2 2 Installing...

Page 105: ...ols and additional items required Screwdriver Torx 10 Solvent suitable for purging Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 To remove harmful substances from the components in the flow path after the purge unit have the pump deliver for a short time with the solvent that was used for purging 3 Turn off the pump flow and wait until the system press...

Page 106: ...mp 7 To attach the mounting bracket for the mixing system inside the pump a Remove the push rivets that cover the mounting holes while no mounting bracket is installed Figure 30 Installation position for mixing system No Description 1 Push rivets covering the mounting holes for the mixing system 2 Mounting position inline filter b Using the screws shipped with the mixing system attach the mounting...

Page 107: ...tatic mixer Figure 32 Static mixer 9 Connect the capillary mixer from the mixer kit to the port labeled OUT on the purge unit and to the static mixer inlet 10 On the static mixer outlet reconnect the capillary to the autosampler injection valve Figure 33 Mixing system installed and connected No Description 1 Capillary mixer on purge unit OUT port 2 Capillary mixer on static mixer inlet 3 Capillary...

Page 108: ... port if applicable 14 Apply the flow rate of your application and have the pump deliver for a short time 15 In Chromeleon a Set StaticMixer to the value indicated on the static mixer b Consider updating the Predictive Performance information InlineFilterChanged command see section 7 4 4 page 126 16 Inspect the flow connections on the mixing system for indications of leakage before resuming operat...

Page 109: ...lvent shut off valve kit containing one shut off valve and fitting connections Tools and additional items required Tubing cutter Solvent suitable for purging the pump Preparations Turn off the pump flow and wait until the system pressure is down to zero Follow these steps Follow the steps for each solvent line in which you want to install a shut off valve 1 Unscrew the cap of the solvent reservoir...

Page 110: ...tion of the fitting and ferrule see picture c Tighten the solvent lines to the shut off valve Figure 34 Installing a shut off valve in a solvent line No Description 1 Solvent line 2 Fitting 3 Ferrule 4 Shut off valve Figure 35 Solvent line with shut off valve installed 6 Check the solvent lines across the entire flow path Make sure that the lines are not bent pinched or squeezed at any point in th...

Page 111: ...y your system arrangement For your Vanquish detector note the following Charged aerosol detector Check that sufficient gas is available to continue gas flowing through the detector This is to prevent any build up of residue from solvents or analytes Gas must be flowing when pump flow is delivered to the detector UV VIS detector The lamp s in the detector can remain turned on The shutter can be mov...

Page 112: ...ith methanol 100 acetonitrile should not be used If an additive is used flush the system with several volumes for example 1 0 mL min for 10 minutes with the standard system of methanol and water 50 50 to prevent salt buildup in the fluidics If the solvents in the pump are not miscible with water use an appropriate intermediate solvent Pump shall be transported or shipped after shutdown If no addit...

Page 113: ...ul not to pull on other tubing in the guides 5 Empty the seal wash system a Unscrew the cap of the seal wash reservoir and remove the seal wash line together with the cap from the seal wash reservoir b Remove the peristaltic tubing from the seal wash pump When the tubing is removed any liquid that is present in the seal wash line flows off to waste 6 The step depends as follows Pump and all other ...

Page 114: ...hole in the reservoir cap If it does not press the retaining guide into the hole to ensure that the tubing is kept in place in the cap 3 Turn on the pump 4 Prepare and restart the other modules in the Vanquish system following the instructions in the Operating Manuals for the modules Pay special attention to the Preparing the Module for Operation section 5 Purge the pump and then have the pump del...

Page 115: ... Maintenance and Service Vanquish Pumps VH P10 A 02 Operating Manual Page 115 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform ...

Page 116: ...ity referred to as Thermo Fisher Scientific service personnel The pump is designed for easy maintenance and service The user serviceable parts of the pump can be accessed from the front If not stated otherwise the maintenance procedures do not require that you remove the pump from the system The maintenance procedures do not require that you remove the doors However it is possible to remove a door...

Page 117: ...NING Tilting Liquid Reservoirs Liquids in the reservoirs on the solvent rack might contain harmful substances Spilling of these substances can pose health and safety risks To prevent the reservoirs from tilting be careful not to pull on the liquid lines when performing maintenance WARNING Escape of Hazardous Substances from Flow Connections Flow and capillary connections can be filled with substan...

Page 118: ...rostatic Pressure Solvent may spill when you open the flow path This is due to hydrostatic pressure in the system when the solvent reservoirs are located above the pump outlet Before you loosen a connection in the flow path turn off the pump flow and wait until the system pressure is down to zero Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from th...

Page 119: ...ed for the procedure Before opening the flow path to replace capillaries in the system turn off the pump flow and wait until the system pressure is down to zero Dirty components can contaminate the chromatography system Contamination leads to poor performance of the modules and entire system or can even cause damage to the modules and system Therefore Always wear appropriate gloves Place the compo...

Page 120: ...eous buffer replace the solvent in the reservoir on a daily basis Check the liquid level in the seal wash reservoir Fill the seal wash reservoir with fresh seal wash liquid if required When you use buffers or salt solutions flush the pump thoroughly after use with an appropriate solvent that does not contain buffers or salts Regularly Replace the wash liquid in the seal wash reservoir with fresh l...

Page 121: ...ns of damage for example scratches or scores see section 7 7 4 page 145 Have Thermo Fisher Scientific service personnel perform preventive maintenance once a year A maintenance kit is available for the pump which includes all parts that are required for routine maintenance see section 10 4 Consumables and Replacement Parts page 223 TIP The Chromeleon software supports functions for estimating the ...

Page 122: ...igh quality solvents minimum UHPLC grade or LC MS grade that are replaced frequently Regularly flushing the degassing channels with fresh solvent is usually sufficient If you use water acetonitrile or methanol for your chromatography flushing the channels once a week is an appropriate interval Mobile phases containing salts buffering agents and or other additives are inherently prone to organic co...

Page 123: ... for collecting the nitric acid Fresh water HPLC grade Fresh acetonitrile HPLC grade New solvent reservoirs with fresh solvent New frits for solvent line filters Follow these steps 1 Turn off the pump flow and wait until the system pressure is down to zero 2 Install the backpressure capillary on the pump outlet Guide the free end of the capillary to the waste container 3 Replace the frits in the s...

Page 124: ...or Decontaminating the Pump Cleaning and decontamination must be performed by qualified personnel wearing suitable personal protective equipment Always observe national and local regulations NOTICE Wipe up all liquids spilled onto the system immediately If surfaces are exposed for longer periods these liquids can cause damage Decontamination Decontamination is required for example when leakage or ...

Page 125: ...at will not damage the surfaces of the system Never use sharp tools or brushes for cleaning any surfaces Do not use sprays for cleaning Prevent cleaning detergent from entering the flow path Do not use excessively wetted cloth or wipes for cleaning Prevent any liquids from entering the functional components of the pump Liquids can cause a short circuit when getting in contact with the electronic c...

Page 126: ...you before and when the replacement service or qualification is due Color coded bars on special panels provide visual feedback allowing you to easily check and monitor the status If a warning limit was set a message in the Chromeleon Audit Trail alerts you when the action is due Some counters can be reset to zero after the required action was performed To keep the Predictive Performance informatio...

Page 127: ...P10 A 02 Operating Manual Page 127 PistonsChanged For each pump head a common counter is available for both pistons SealsChanged For each pump head a common counter is available for all piston seals in the pump head QualificationDone ServiceDone ...

Page 128: ... detector drop detector see section 7 5 3 page 132 TIP To replace the seal wash seal which are part of the pump head see section 7 7 7 page 153 7 5 1 Testing the Seal Wash System for Leakage When At regular intervals If you performed maintenance on components in the pump head for example after replacing the piston seals Items required Syringe 12 mL Follow these steps 1 Disconnect the tubing from t...

Page 129: ...ons of the seal wash system or under the pump heads If leakage is present tighten or replace leaky connections as necessary and repeat the inspection If no leakage is present remove the syringe and reconnect the tubing to the seal wash detector port labeled in 7 5 2 Replacing the Seal Wash Lines When Damage or blockage of the seal wash lines Parts and additional items required Seal wash lines as r...

Page 130: ...mpty Follow these steps 1 When you have to remove a seal wash line from the tubing guides be careful not to pull on other tubing in the guides 2 Follow the sequence of steps for the seal wash line that you want to replace Seal wash lines that connect the seal wash reservoir to the peristaltic tubing in the pump Follow the steps in the Operating Manual for the Vanquish autosampler Peristaltic tubin...

Page 131: ...hand insert the peristaltic tubing between the lever and the rotor and wind it around the rotor g Release the lever h Insert the peristaltic tubing into the left tubing holder i Check that the peristaltic tubing is properly inserted in the tubing holders If it is not push the tubing into the holders Make sure that the tubing is not pinched or squeezed in the holders j Continue with the next step S...

Page 132: ...ction 5 6 6 3 page 75 Use fresh seal wash liquid 7 5 3 Replacing the Seal Wash Detector When Contamination of detector electrodes Impaired functionality of the seal wash system Parts required Seal wash detector assembly consisting of the detector and funnel Follow these steps Figure 38 Removing the seal wash detector assembly 1 Disconnect the tubing from the seal wash detector port labeled in 2 Un...

Page 133: ...h parts for any reason push the detector into the funnel Mind the orientation of the funnel b Push the assembly into the pump Figure 39 Installing the seal wash detector assembly No Description 1a Funnel bottom side 1b Funnel top side 2 Seal wash detector 5 Reconnect the tubing to the seal wash detector port labeled in 6 Connect the detector cable to the Drop Det port 7 Flush the seal wash system ...

Page 134: ...s 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps Figure 40 Y Connector used as left Y connector A or right Y connector B No Description 1 Capillaries to outlet check valves on pump head 2 Capillary to purge unit port marked R or L depending on Y connector position 1 Disconne...

Page 135: ...irst connect the Y connector capillaries to the outlet check valves Then connect the capillary to the purge unit 3 Purge the pump using the solvent for your application 4 Inspect the flow connections of the Y connector for indications of leakage Tighten leaky connections when the system pressure is down to zero ...

Page 136: ...7 2 page 138 To replace a piston see section 7 7 3 page 142 To clean a piston see section 7 7 4 page 145 To replace the piston seals or support ring see section 7 7 5 page 146 To replace a seal wash seal see section 7 7 7 page 153 To replace the pump head seals see section 7 7 8 page 158 To test the pump for piston seal leakage see section 7 7 9 page 161 For an overview of the pump head parts see ...

Page 137: ...Figure 41 Overview of pump head parts No Description 1 Inlet assembly 2 Inlet check valves 3 Pump head 4 Pump head seals 3 PTFE O ring seals 5 Piston seals 6 Support rings 7 Seal wash bodies pump head bushing 8 Seal wash seals 9 Seal wash plate 10 Screws seal wash plate 8 screws 11 Pistons 12 Connectors for seal wash tubing screwable 13 Outlet check valves ...

Page 138: ...flow and wait until the system pressure is down to zero Follow these steps to remove the pump head 1 Select the pump head for which you want to perform the procedure 2 Undock the pistons either from the keypad or from Chromeleon Wait until undocking is complete before proceeding with the next step 3 On the inlet assembly of the pump head disconnect the tubing from the degasser 4 Unplug the cable o...

Page 139: ...allation of pump heads Be sure not to interchange the pump heads To identify the correct installation position note the labeling on the pressure sensor cable L for the left pump head or R for the right pump head If the pistons remained in the pump head consider verifying that the distance between the pistons and seal wash plate is correct see the related steps in section 7 7 3 page 142 If you have...

Page 140: ... 134 5 Connect the cable of the pressure sensor to the P Work connector 6 On the inlet assembly of the pump head connect the tubing from the degasser 7 If the pump is connected in Chromeleon disconnect the pump 8 Turn off the pump with its main power switch and on again 9 Dock the pistons either from the keypad or from Chromeleon Wait until docking is complete before proceeding with the next step ...

Page 141: ...al Page 141 a From Chromeleon run the pump specific Performance Qualification tests PQ tests Flow Precision Test and Gradient Accuracy Test For details refer to the Operational and Performance Qualifications manual b In Chromeleon execute the QualificationDone command ...

Page 142: ...ue which is imprinted on the rear of the piston see picture and the installation position in the pump head left or right You might need this assignment in a later step Figure 43 Piston calibration value Reinstallation of pistons Be sure not to interchange the pistons Installation of new pistons replacement pistons New pistons can be installed in either position 3 When reinstalling a piston you rem...

Page 143: ... pistons 6 Place the spacing tool onto the pump head Figure 45 Installing the spacing tool and pistons No Description 1 Spacing tool 2 Pistons 7 Push in the pistons until they contact the tool 8 Remove the spacing tool Be careful not to pull out the pistons any further 9 Install the pump head Follow the related steps in section 7 7 2 page 138 ...

Page 144: ...aseline fluctuation or pulsation When reinstalling a piston Consider verifying that the Piston Calibration Value property for the related piston matches the value on the piston Non matching values may result in periodic baseline fluctuation or pulsation 11 Inspect all flow connections on the pump head for indications of leakage Tighten leaky connections when the system pressure is down to zero 12 ...

Page 145: ...n preferably using isopropanol 3 With a dry lint free tissue rub the piston several times 4 Recommended Even if the piston seems to be clean perform the fingernail test a Hold the piston and carefully move your fingernail over the piston surface The piston is clean when the fingernail test does not reveal inconsistent or rough areas b Repeat steps 2 and 3 5 When no damage is visible and the piston...

Page 146: ...support ring is not a wear part You need not replace the support ring when you replace a piston seal Replace the support ring only when applicable Optional Seal wash seals and seal handling tool Replacing the seal wash seal is recommended Tools and additional items required Screwdriver Torx TX25 Seal handling tool Figure 46 Seal handling tool No Description 1 Seal removal pin 2 Seal insertion pin ...

Page 147: ...p head Repeat on the other side Figure 47 Removing the seal wash plate with the spacing tool 3 Remove the seal wash bodies from the pump head if applicable 4 Remove the support ring Gently tap the pump head on a clean work surface to make the support ring drop out If the support ring does not drop out use the seal handling tool With the insertion pin push the seal handling tool at a slight angle i...

Page 148: ...ing from the inside and outside using isopropanol a cleaning swab and a lint free tissue 7 Pipette a few drops of isopropanol into cavity in the pump head on the edge on which the piston seal will rest Figure 48 Edge on which the piston seal will rest 8 First slide the support ring onto the insertion pin of the seal handling tool Then slide the piston seal onto the insertion pin with the open side...

Page 149: ...ring remain in the pump head Figure 50 Pump head piston seals and support rings installed 11 Insert the seal wash bodies into the pump head if applicable Mind the correct orientation The even side faces the pump head Figure 51 Orientation of the seal wash body No Description 1 Side facing the pump head 2 Side facing the seal wash plate 12 Recommended Replace also the seal wash seals Follow the app...

Page 150: ...ews step by step in the given order once again 16 Install the pistons Follow the related steps in section 7 7 3 page 142 17 Install the pump head Follow the related steps in section 7 7 2 page 138 18 Observe the recommendations for new piston seals see section 7 7 6 page 151 19 Inspect all flow connections on the pump head for indications of leakage Tighten leaky connections when the system pressu...

Page 151: ...ded in this section directly influence pump performance and the piston seal life cycle Items required nanoViper capillary length 750 mm inner diameter 75 µm Isopropanol Solvent suitable for your application Recommendations Allow new piston seals to run in To run in new piston seals follow these steps 1 Purge the pump For 15 minutes apply a purge flow of approximately 1 3 mL min using isopropanol 2...

Page 152: ...mp using the solvent for your application 7 If an increased leakage rate is observed with new piston seals after several hours of operation operate the pump for at least 2 hours at 35 MPa TIP If the piston seals are not sufficiently tight yet have the pump build up and hold high pressure for some minutes Never run the pump dry Running the pump dry can damage the pistons or piston seals ...

Page 153: ... 146 Syringe filled with isopropanol Preparations 1 Remove the pump head see section 7 7 2 page 138 2 Remove the pistons see section 7 7 3 page 142 Follow these steps 1 Remove the screws on the rear of the pump head on the seal wash plate 2 Remove the seal wash plate from the pump head TIP Removing the seal wash plate may be easier with the spacing tool Insert the spacing tool into the opening on ...

Page 154: ...pacing tool Figure 55 Pump head seal wash plate and pistons removed No Description 1 Pump head 2 Seal wash plate channel side 3 Seal wash seals 4 Pistons 3 If the seal wash bodies are present in the seal wash plate remove them from the plate No Description 1 Pump head 2 Opening on seal wash plate 3 Spacing tool 4 Seal wash plate ...

Page 155: ...insertion pin of the seal handling tool 6 To facilitate seal installation inject a few drops of isopropanol into the seal cavities on the seal wash plate no channel side 7 On the seal wash plate no channel side set the insertion pin of the seal handling tool at a 90 angle to the plate and push the insertion pin vertically into the plate as far as it goes in 8 Remove the tool from the seal wash pla...

Page 156: ...head Figure 58 Orientation of the seal wash body No Description 1 Side facing the pump head 2 Side facing the seal wash plate 10 Place the seal wash plate onto the pump head and insert the screws Figure 59 Pump head with seal wash plate and plate screws 11 With the screwdriver hand tighten the screws step by step in the order shown in the picture Figure 60 Tightening order for seal wash plate scre...

Page 157: ... the pump head Follow the related steps in section 7 7 2 page 138 15 Inspect all flow connections on the pump head for indications of leakage Tighten leaky connections when the system pressure is down to zero 16 Test the seal wash system and the pump for leakage 17 If you replaced the seal wash seals together with the piston seals proceed with the specific steps required after piston seal replacem...

Page 158: ...page 142 Follow these steps 1 Remove the screws on the rear of the pump head on the seal wash plate 2 Remove the seal wash plate from the pump head TIP Removing the seal wash plate may be easier with the spacing tool Insert the spacing tool into the opening on one side of the seal wash plate and lever the plate away from the pump head Repeat on the other side Figure 61 Removing the seal wash plate...

Page 159: ...1 Pump head seal O ring 65x1 5 2 Pump head seal O ring 9x1 5 3 Pump head seal O ring 45x1 5 5 Install the new seals Place the seal onto the receiving groove With your finger on the seal follow the groove shape until the seal rests exactly in the groove 6 Insert the seal wash bodies into the pump head if applicable Mind the correct orientation The even side faces the pump head Figure 63 Orientation...

Page 160: ...n the picture Figure 65 Tightening order for seal wash plate screws 9 Tighten the screws step by step in the given order once again 10 Install the pistons Follow the related steps in section 7 7 3 page 142 11 Install the pump head Follow the related steps in section 7 7 2 page 138 12 Inspect all flow connections on the pump head for indications of leakage Tighten leaky connections when the system ...

Page 161: ...Chromeleon 6 8 and Chromeleon 7 earlier than Chromeleon 7 2 6 Parts and additional items required Backpressure capillary from Diagnostics tool kit optional Isopropanol Follow these steps 1 From Chromeleon start a seal wash cycle 2 Disconnect the tubing from the seal wash detector port labeled in Figure 66 Seal wash detector 3 Remove some liquid from the tubing for example by shaking the tubing 4 S...

Page 162: ...ing is increasing One or more piston seals are leaky Continue with steps 8 to end 8 On the right pump head remove the seal wash tubing that connects the left pump head to the right pump head 9 Apply a flow rate of 3 mL for 100 solvent A using isopropanol 10 For about 5 minutes observe the liquid level in the tubing Finish the observation before a new seal wash cycle starts 11 Depends on your obser...

Page 163: ...alves The picture shows the parts that must be removed Figure 67 Replacing the inlet check valves When Damage of check valve or leaky check valve Parts required Inlet check valve Additional items required Solvent suitable for purging to flush out harmful substances Solvent suitable for your application Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Turn...

Page 164: ...the assembly 3 Loosen and remove the inlet check valve 4 Tighten the new inlet check valve finger tight 5 Install the inlet assembly a Push the assembly onto the locating pin b Set the lever on the assembly into the Lock position 6 On the inlet assembly reconnect the tubing from the degasser 7 Purge the pump using the solvent for your application 8 Inspect the check valves and inlet assembly conne...

Page 165: ... flush out harmful substances Solvent suitable for your application Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Turn off the pump flow and wait until the system pressure is down to zero 3 Remove the Y connector see section 7 6 page 134 Follow these steps 1 Loosen and remove the outlet check valve 2 Tighten the new outlet check valve finger tight 3 In...

Page 166: ... to clean It is recommended to clean all check valves of the pump head at the same time To remove the check valves follow the appropriate steps in section 7 8 1 page 163 inlet check valves or section 7 8 2 page 165 outlet check valves Follow these steps 1 In the direction of flow fill the check valves with isopropanol using the syringe 2 For 10 minutes place the check valves in the ultrasonic bath...

Page 167: ...f you observe pressure pulsation inconstant pressure or high backpressure at the column and pump Additional items required Water as solvent Tissue Follow these steps 1 Disconnect the capillary on the outlet of the inline filter 2 To absorb the liquid leaving the filter place the tissue under the filter outlet 3 Apply a flow of 2 mL min using water 4 Observe the pump pressure 5 Depends on the press...

Page 168: ...bstances from the components in the flow path after the purge unit have the pump deliver for a short time with the solvent that was used for purging 3 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Unplug the cable of the seal wash detector from the DROP DET port 2 Unplug the cable of the right pump head from the P WORK port if applicable 3 Remove th...

Page 169: ...ow on the capillary mixer 9 On the filter outlet reconnect the capillary to the autosampler injection valve 10 Reinstall the right Y connector 11 Reconnect the cable of seal wash detector to the DROP DET port 12 Reconnect the pump head cable to P WORK port if applicable 13 Apply the flow rate of your application and have the pump deliver for a short time 14 Inspect the flow connections on the inli...

Page 170: ...ion inconstant pressure or high backpressure at the column and pump Additional items required Water as solvent Tissue Follow these steps 1 Disconnect the capillary on the outlet of the static mixer 2 To absorb liquid leaving the static mixer place the tissue on the mixer outlet 3 Apply a flow of 2 mL min using water 4 Observe the pump pressure 5 Depends on the pressure reading Pressure is less tha...

Page 171: ...urge unit have the pump deliver for a short time with the solvent that was used for purging 3 Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Unplug the cable of the seal wash detector from the DROP DET port 2 Unplug the cable of the right pump head from the P WORK port if applicable 3 Remove the right Y connector see section 7 6 page 134 4 As applica...

Page 172: ...ted by the arrow on the capillary mixer 9 On the static mixer outlet reconnect the capillary to the autosampler injection valve 10 Reinstall the right Y connector 11 Reconnect the cable of seal wash detector to the DROP DET port 12 Reconnect the pump head cable to P WORK port if applicable 13 Apply the flow rate of your application and have the pump deliver for a short time 14 Inspect the flow con...

Page 173: ...ssure is down to zero Follow these steps Follow these steps for each solvent line that you want to empty 1 Unscrew the cap of the solvent reservoir and remove the solvent line together with the cap from the reservoir 2 Start a purge cycle as appropriate 3 Stop purging as soon as the solvent line is empty 7 11 2 Replacing Solvent Lines To replace solvent lines follow the sequence of steps for the s...

Page 174: ...or purging Preparations 1 To flush out harmful substances purge the pump with an appropriate solvent 2 Empty the solvent lines see section 7 11 1 page 173 Follow these steps 1 Remove the solvent line from the reservoir cap a Remove the filter from the solvent line b Remove the retaining guide c Pull the solvent line out of the reservoir cap 2 Disconnect the solvent line on the pump inlet 3 Remove ...

Page 175: ...e on the pump inlet and on the degasser 2 Install the new solvent line 3 To remove any air bubbles from the solvent lines purge the pump 7 11 2 3 Solvent Line From Degasser To Pump Head When Damage or blockage of the solvent line Parts and additional items required Solvent line between degasser and pump head inlet Solvent suitable for purging Preparations To flush out harmful substances purge the ...

Page 176: ...g Preparations Turn off the pump flow and wait until the system pressure is down to zero Follow these steps 1 Unscrew the cap of the solvent reservoir and remove the cap together with the solvent line from the reservoir 2 Remove the filter holder from the solvent line 3 As applicable Open the filter holder and remove the filter frit 4 Assemble the solvent line filter wearing appropriate clean glov...

Page 177: ... onto the solvent line 6 Retighten the reservoir cap hand tight Verify that the retaining guide remains in the hole in the reservoir cap If it does not press the retaining guide into the hole to ensure that the tubing is kept in place in the cap 7 To remove any air bubbles from the solvent lines purge the pump No Description 1 Solvent line filter bottom 2 Filter frit 3 Solvent line filter top ...

Page 178: ...he outlet of the inline filter or static mixer if installed 2 Close the outlet with the fitting plug 3 Set the upper pressure limit to 145 MPa 4 Apply a flow of 50 µL min and use the solvent for the application 5 Observe the pressure When the pressure reading is between 60 MPa and 100 MPa change the flow rate to a lower value 6 Have the pump deliver until a pressure of 140 MPa has built up 7 When ...

Page 179: ...n the connection port turn off the pump flow When the system pressure is down to zero tighten the check valve a little more Remove the check valve Clean the check valve in an ultrasonic bath If cleaning is not sufficient replace the check valve Purge Unit Pull the waste line out of the waste outlet port see Figure 13 page 64 and repeat the test If liquid leaves the waste line the purge unit is lea...

Page 180: ...resent inside the pump that could cause an electric shock or damage to the device Turn off the pump with its main power switch Disconnect the power cord from both the power source and the device Use only the fuses of the type and current rating specified for the pump by Thermo Fisher Scientific Do not use repaired fuses and do not short circuit the fuse holders Follow these steps The fuse holder i...

Page 181: ... the screwdriver to remove the fuse holder 2 Replace the two fuses with new fuses of the specified type and current rating Always replace both fuses 3 Reinstall the fuse holder 4 Reconnect the power cord to the power source and to the pump 5 Turn on the pump with the main power switch ...

Page 182: ...ed automatically to the device when you install the Chromeleon Service Release Preparations Read the release notes provided with the firmware and or Chromeleon Service release Verify the following The pump is connected in Chromeleon All operations on the instrument Chromeleon 7 or timebase Chromeleon 6 8 that includes the pump have been stopped The instrument or timebase is idle The pump flow is t...

Page 183: ...from the Chromeleon software If the firmware installed in the pump is a later version than the version in Chromeleon cancel the download The firmware update may take several minutes Monitor the Audit Trail of the Chromeleon Instrument Configuration Manager or Server Configuration program to see whether the firmware update was successful or failed If the firmware update failed turn the device off a...

Page 184: ...erforming the following sequence of steps and do not apply force 1 To remove a door push the door upward while opening Open the door to a position in which the two hinges on the housing are aligned in the grooves on the door You can remove the door only when the hinges are in the grooves Figure 71 Unhinging a door 2 Slightly tilt the door to the outside away from the housing and remove the door 3 ...

Page 185: ...quish Pumps VH P10 A 02 Operating Manual Page 185 4 Insert the hinges in the groove by pushing up and slightly turning the door 5 Push the door downward to lock it in place You can close the door only when it is properly installed ...

Page 186: ...lone to handle safely To avoid personal injury or damage to the pump observe the following guidelines Physical handling of the pump including lifting or moving requires a team effort of two persons Use the carrying handles that were shipped with the pump to move or transport the pump Never move or lift the pump by the front doors This will damage the doors or the pump Follow these steps 1 Prepare ...

Page 187: ...et port see Figure 13 page 64 6 Check the leak tray and drain port If liquid is present absorb the liquid with a tissue 7 Remove the pump or slide in module from the system stack as required Install the carrying handles and remove the pump from the Vanquish system Follow the instructions on dismounting the system stack in the Transporting or Shipping the System section of the Vanquish System Opera...

Page 188: ...al b To prepare the pump for first time operation follow the instructions in the Preparing the Pump for Operation section in this operating manual 5 Before starting an analysis let the pump equilibrate and be sure that it is ready for operation 7 16 3 Shipping the Pump To ship the pump follow these steps 1 Follow the unpacking instructions in this manual in the reverse order Use only the original ...

Page 189: ...o accept devices for repair if the Decontamination Certificate is missing Restarting the Pump after Shipping To restart the pump after shipping follow these steps 1 Follow the unpacking instruction in this operating manual 2 Install and set up the pump in the system stack Follow the instructions on mounting the system stack in the Vanquish System Operating Manual 3 Set up the pump a To connect the...

Page 190: ... the pump including lifting or moving requires a team effort of two persons A team effort is in particular required when lifting the pump into the system stack or when removing it 7 17 1 Removing the Slide In Module Tools required Screwdriver Torx T20 Preparations Prepare the pump for transport See the Transporting or Shipping the Pump section in this operating manual Follow these steps 1 Loosen t...

Page 191: ... enclosure too far Pull out the slide in module just far enough so that you can grasp it on both sides from below 4 Remove the slide in module from the enclosure The following steps require a team effort a Take the slide in module on both sides from below b Pull the slide in module from the rails towards the front c Place the slide in module on a clean and stable surface 5 Return the slide in modu...

Page 192: ... device has been properly decontaminated and that it is free of hazardous substances Thermo Fisher Scientific refuses to accept devices for repair if the Decontamination Certificate is missing 7 17 2 Installing the Slide In Module Tools required Screwdriver Torx T20 Preparations 1 Unpack the replacement pump Remove the slide in module from its enclosure as described in the Removing the Slide In Mo...

Page 193: ...le to the height of the enclosure c Place the slide in module in the enclosure so that the module sits in the enclosure by approximately 25 cm d Push the slide in module onto the rails and into the enclosure until the slide in module sits completely in the enclosure Figure 74 Inserting the slide in module 3 Gradually and evenly tighten the four captive screws on the slide in module hand tight NOTI...

Page 194: ...on in this operating manual 5 Prepare all other modules of the Vanquish system for operation and restart them Refer to the Operating Manuals for the modules 6 Before starting an analysis let the chromatography system equilibrate and be sure that it is ready for operation 7 Perform performance qualification for the pump a From Chromeleon run the pump specific Performance Qualification tests PQ test...

Page 195: ...8 Troubleshooting Vanquish Pumps VH P10 A 02 Operating Manual Page 195 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the pump ...

Page 196: ...covered in this section contact Thermo Fisher Scientific Technical Support for assistance See the contact information at the beginning of this manual To facilitate device identification have the serial number and technical name available when communicating with Thermo Fisher Scientific Status Indicators The status indicator LED bar on the front side of the pump and the STATUS LED on the keypad ins...

Page 197: ...e panel tabset Sequence Control panel In addition Daily Audit Trails are available in the Browser in the folder of the timebase Messages in the Chromeleon Audit Trail are preceded by an icon The icon identifies the seriousness of the problem The table shows the icons and explains the severity level Icon Severity Description Warning The queue Chromeleon 7 or batch Chromeleon 6 8 can be started The ...

Page 198: ...dit Trail when the module is connected in Chromeleon In this case observe the following Send the Audit Trail as cmbx file to the Technical Support before you clear the log To clear the exception log and continue operation of the module perform the command ExceptionLogClear For more information refer to the Chromeleon Help Chromeleon Tests With Chromeleon 7 2 6 and later tests are available to help...

Page 199: ...ing text Drive1 Indicates that components on the left side in the pump block head cause the problem Drive2 Indicates that components on the right side in the pump block head cause the problem Example Pump_Wellness_LeftBlock Drive 2 in front of a message indicates that the message refers to the components on the right side in the left pump block head The table lists the most frequently observed mes...

Page 200: ... the leakage reported earlier with code 33 was not resolved within 3 minutes Find and eliminate the source for the leakage see section 8 4 page 208 Code 36 Download failed The firmware download has not been successful Repeat the download Code 37 Download firmware mismatch You tried to download a firmware file that is invalid for the pump Repeat the download with a firmware file that is valid for t...

Page 201: ...e column If the problem occurs due to column ageing it may be sufficient to increase the setting for the upper pressure limit The autosampler may be blocked Check the autosampler components and eliminate the source for the blockage If the flow path is not blocked Depending on the pump configuration test the inline filter or static mixer for permeability depending on which component is installed se...

Page 202: ...12 Self test failed because of fluctuating pressure Changes in pressure have occurred during the self test Wait until the system pressure is down or depressurize the system and repeat the self test Code 4113 Self test failed because pressure is out of range The pressure has not been down to zero during the self test Verify that the pressure sensor cables are properly connected Wait until the syste...

Page 203: ...n the drop counter functionality of the seal wash detector is enabled standard setting Test the pump for piston seal leakage and take the recommend action see section 7 7 9 page 161 Code 4122 The rear seal leak sensor detects drops constantly This message alerts you only when the drop counter functionality of the seal wash detector is enabled standard setting Inspect the electrodes of the seal was...

Page 204: ... if you are monitoring the liquid level in the waste container see section 6 8 page 98 Empty the waste container when needed Code 4161 Pressure recalibration deviates by xx bar The pump pressure has not been down to zero during the self test Verify that the pressure sensor cable is properly connected to the P Sys connector Wait until the pressure is down and repeat the test Code 4176 The pressure ...

Page 205: ...place leaky components as necessary Code 4212 Nonlinear pressure sensor Please perform pressure recalibration Start the pressure recalibration procedure see section 8 5 3 page 213 Code 4213 Head pressure does not match system pressure signal Execute self test or perform pressure recalibration First run the self test If the message appears again perform pressure recalibration see section 8 5 3 page...

Page 206: ...socket XX is the left pump head or right pump head or the system pressure sensor You may have connected the cable of the device indicated in the message to the wrong socket for example the cable of the system pressure sensor has been connected to the socket for the pump head cable Verify that the cable is connected to the correct socket and that the cable is properly seated Code 4234 The flow was ...

Page 207: ...eak Test checks the capillary and fitting connections Pressure pulsation shifts in retention time The Advanced Leak Test checks the check valves piston seals and capillaries Parts required Fitting plug Solvent of your application Follow these steps 1 In Chromeleon on the Wellness sub panel for the pump start the test that you want to run 2 A wizard guides you through the process Follow the instruc...

Page 208: ...sented in chapter 7 Maintenance and Service page 115 As leakage usually occurs at a connection inspect all components and connections in the flow path Parts required Replacement part as required Additional items required Cloth or tissue Follow these steps 1 Locate the source of the leak The following tests can help you to identify the source Test the seal wash system for leakage see section 7 5 1 ...

Page 209: ... Troubleshooting Vanquish Pumps VH P10 A 02 Operating Manual Page 209 4 Allow the sensor to adjust to the ambient temperature for a few minutes 5 If leakage is no longer reported you can resume operation ...

Page 210: ...ections help you to identify and eliminate the source for the problem Pressure pulsation See section 8 5 2 page 211 and section 8 5 4 page 214 Shift in retention times See section 8 5 2 page 211 Messages regarding the pump head pressure or nonlinear pressure sensor appear in chromatography software See section 8 5 3 page 213 Inconstant pressure or high backpressure at the column and pump See secti...

Page 211: ...sionDrv1 compression on the right side in the pump head CompressionDrv2 The values indicate the compression of the last stroke in percent The compression value should be less than 100 With isocratic conditions the compression value should remain stable When running a gradient the compression value changes roughly proportional to the pressure curve If the value is close to 100 the required precompr...

Page 212: ...ression limit reached and low pressure Air bubbles may be present in the pump Make sure that no air bubbles are present in the solvent lines and then purge the pump If solvent shut off valves are installed Verify that the valves are open Excessive leakage may be present on the inlet check valve Remove the solvent line from the solvent reservoir and the solvent line filter from the solvent line Whi...

Page 213: ...re recalibration Follow these steps 1 Before you perform pressure recalibration check the message in the Messages section in this manual Before pressure recalibration should be performed message specific remedial actions might be required Perform these actions as applicable and proceed with the next step 2 On the purge unit remove the capillary from the OUT port 3 Close the port and the open end o...

Page 214: ...lsation inconstant pressure or high backpressure at the column and pump Additional items required Water as solvent Tissue Follow these steps The pump has an inline filter installed Test the inline filter by following the steps in section 7 9 1 page 167 The pump has the mixing system installed Test the static mixer by following the steps in section 7 10 1 page 170 ...

Page 215: ...ons Vanquish Pumps VH P10 A 02 Operating Manual Page 215 9 Specifications This chapter provides the physical and performance specifications including information about the materials used in the flow path of the pump ...

Page 216: ... SD Number of solvents 2 out of 6 Maximum stroke volume 120 µL Mixer volume Standard configuration 25 µL capillary mixer proprietary Other mixing systems are available as an option see section 6 9 2 page 102 Dwell volume 35 µL with standard pump configuration 35 µL to 400 µL with optionally available mixing systems Solvent degassing Built in number of channels 2 out of 6 Biocompatible Yes Communic...

Page 217: ...hnical literature Solvent and additive information See section 2 4 page 27 Safety features Leak detection and safe leak handling excess pressure monitoring Good Laboratory Practice GLP features Predictive Performance functions for scheduling maintenance procedures based on the actual operating and usage conditions of the pump All system parameters logged in the Chromeleon Audit Trail Typical opera...

Page 218: ...ng temperature 5 C 35 C Ambient storage temperature 20 C 45 C Ambient operating humidity 20 80 relative humidity non condensing Ambient storage humidity Maximum 60 relative humidity non condensing Pollution degree 2 Power requirements 100 240 VAC 50 60 Hz max 525 W 550 VA Overvoltage category II Emission sound pressure level 70 dB A typically 48 dB A Dimensions height x width x depth 19 2 cm x 42 ...

Page 219: ...g Manual Page 219 10 Accessories Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the pump and the accessories that are available as an option This chapter also provides information for reordering consumables and replacement parts ...

Page 220: ...e replacement parts and additional components options and peripherals specifically authorized and qualified by Thermo Fisher Scientific Accessories consumables and replacement parts are always maintained at the latest technical standard Therefore part numbers are subject to change If not otherwise stated updated parts will be compatible with the parts they replace ...

Page 221: ...t line filter filter holder no filter frit included 7 Solvent line filter filter frit biocompatible 10 µm 12 Plugs and retaining guides for reservoir caps kit including Cap plug to close open holes in the reservoir cap pack of 5 Retaining guide to keep the liquid line in place in the reservoir cap pack of 2 7 Fitting plug Viper 1 Tool kit including Screwdriver Torx TX25 Hexagon wrench size 6 Seal ...

Page 222: ...les interfere with the detection For TFA applications NOTE The mixer kit includes the mixing system and required installation material Each mixing system comprises a static mixer and a capillary mixer The volumes of both mixers determine the entire volume of the mixing system Mixer kit 200 µL The kit includes the mixing system volume 200 µL comprising Static mixer volume 150 µL Capillary mixer vol...

Page 223: ...ength 5 m 6000 1004 USB cables type A to type B high speed USB 2 0 Cable length 1 m Cable length 5 m 6035 9035 6911 0002 System interlink cable 6036 0004 Maintenance kit Description Part No Maintenance kit including Pump head seal 3 PTFE seals different sized Silicone tubing clear tubing Peristaltic tubing white tubing Tubing connector straight Tubing connector 90 angled Tubing connector ID 1 16 p...

Page 224: ...re capillary length 950 mm 6044 0100 Fitting plug Viper biocompatible 6040 2303 Fuses kit Vanquish system The kit includes the appropriate fuses for the Vanquish system modules For the pump use only the 5AT 230V AC slow blow fuses 6036 0002 Front door kit including right door and left door 6044 1920 Packaging material for pump 6044 7001 Y connector To be used as left Y connector or right Y connect...

Page 225: ...044 1210 Screws for seal wash plate pack of 8 6000 0036 Seal handling tool 6040 7158 Reservoirs for solvents and wash liquids Description Part No Reservoir 1 L including cap 2270 0012 Reservoir 0 25 L including cap 2270 0026 Cap for reservoirs screw cap pack of 4 6270 0013 Plugs and retaining guides for reservoir caps kit including Cap plug to close open holes in the reservoir cap pack of 10 Retai...

Page 226: ... tubing connectors in the autosampler Use the thin silicone tubing and related tubing connectors in the pump Use the screwable connectors for the pump head 6044 1150 Piston seal RP and seal wash seal pack of 2 each 6266 0309 Solvent lines solvent line filters waste tubing Description Part No Solvent lines to connect the solvent reservoirs to the pump inlet 6036 1701 Tubing kit including Solvent li...

Page 227: ...11 Appendix Vanquish Pumps VH P10 A 02 Operating Manual Page 227 11 Appendix This chapter provides additional information about compliance and the use of the digital I O ports ...

Page 228: ...zardous substances in electrical and electronic equipment The CE mark on the device indicates that the product is compliant with the directive China RoHS regulations Measures for Administration of the Pollution Control of Electronic Information Products One of the following logos may be present on the device if applicable Logo Description The green logo marks items that do not contain the hazardou...

Page 229: ...Class A digital device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications...

Page 230: ...y_COM COM is the common contact for NO and NC If the relay is not activated or if the pump is turned off the connection is between COM and NC If the relay is activated the connection is between COM and NO 6 Relay output Relay_NO Normally Open contact To use the digital I O functionality the following prerequisites must be fulfilled The digital I O port must be connected to the external device with...

Page 231: ...erence to ground The minimum input voltage must not be lower than the ground potential 5 White Relay output Relay_COM Potential free Common contact for NO and NC 6 Brown Relay output Relay_NO Potential free 0 24 V 0 100 mA Closing contact 2 For each relay output or digital input to be used connect the appropriate signal wire and ground wire to the corresponding connectors on the external device Fo...

Page 232: ...11 Appendix Page 232 Vanquish Pumps VH P10 A 02 Operating Manual ...

Page 233: ...12 Index Vanquish Pumps VH P10 A 02 Operating Manual Page 233 12 Index ...

Page 234: ...tup 79 Simple Leak Test 207 Smart Shutdown 111 Smart Standby 111 Smart Startup 91 clean pump 123 surface 124 compression 93 compression values 211 condensation 52 connectors 56 consumables 219 223 cTUVus mark 228 curve 93 D decontamination 124 degasser mode 93 Dig I O 57 230 digital I O 57 230 Dock button 85 door replace 184 unhinge 116 184 drain port 39 64 drain system 64 drainage 64 drop detecto...

Page 235: ...g principle 33 operation 40 81 interrupt 111 long term shutdown 112 short term shutdown 111 outlet check valve clean 166 replace 165 overview functional 31 P peristaltic tubing replace 129 permeability inline filter 167 static mixer 170 pH range 27 piston clean 145 replace 142 piston calibration value 94 piston seal leak test 161 replace 146 run in 151 piston seal wash cycle 94 piston seal wash sy...

Page 236: ...8 SealsChanged 127 Select button 85 Select LEDs 85 self test 78 88 service 115 rules 119 safety 117 ServiceDone 127 setup flow connections 59 hardware 54 software 79 ship 186 ship kit 221 short term shutdown 111 shutdown long term 112 short term 111 signal words 18 Simple Leak Test 207 slide in module install 192 remove 190 Smart Shutdown 111 Smart Standby 111 Smart Startup 91 solvent chloride con...

Page 237: ...utton 88 T TFA 101 transport 186 troubleshooting 195 check compression values 211 messages 199 resolve leakage 208 tubing install 62 tubing bracket 60 tubing chase 60 tubing guides 60 type label 20 U Universal Serial Bus 57 unpack 42 USB 57 V Viper 62 W waste 64 waste level monitoring 97 waste outlet port 64 WEEE 229 Y Y connector maintenance 134 replace 134 ...

Page 238: ...Thermo Fisher Scientific Inc 168 Third Avenue Waltham Massachusetts 02451 USA www thermofisher com ...

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